ZAP70 Antikörper (pTyr493)

Produktnummer ABIN498719

Der Kaninchen Polyklonal anti-ZAP70 Antikörper wird verwendet zum Nachweis von ZAP70 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IF und IHC (p).

Kurzübersicht für ZAP70 Antikörper (pTyr493) (ABIN498719)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ZAP70 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ZAP70 Antikörper

Bindungsspezifität: pTyr493

Spezifität: This antibody detects endogenous levels of ZAP-70 protein only when phosphorylated at Tyr493.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Andere Bezeichnung: ZAP70

Hintergrund: The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti alpha and beta chains, the CD3 gamma, delta and epsilon chains and a zeta-containing homodimer or heterodimer. The protein tyrosine kinase ZAP-70 binds to the phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCR zeta chain through two Src homology (SH2) domains. This binding results in the phosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr 292 and Tyr 319. ZAP-70 is autophosphorylated on Tyr 292, which is thought to negatively regulate ZAP-70 function in lymphocytes. Alternatively, ZAP-70 is positively regulated by phosphorylation on Tyr 319, which mediates the SH2-dependent interaction between Lck and ZAP-70.Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZAP 70, ZAP-70

Molekulargewicht: approx. 70 kDa

Gen-ID: 7535

NCBI Accession: NP_001070

UniProt: P43403

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway