PRKAG1 Antikörper

Produktnummer ABIN498427

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch PRKAG1 in WB und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für PRKAG1 Antikörper (ABIN498427)

Target: PRKAG1 (Protein Kinase, AMP-Activated, gamma 1 Non-Catalytic Subunit (PRKAG1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKAG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-PRKAG1 Antikörper

Spezifität: This antibody detects endogenous levels of AMPKgamma1/2/3 protein. (region surrounding Lys78)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu PRKAG1

Target: PRKAG1 (Protein Kinase, AMP-Activated, gamma 1 Non-Catalytic Subunit (PRKAG1))

Andere Bezeichnung: AMPK gamma-1 Chain / AMPKg

Hintergrund: The 5-AMP activated protein kinase (AMPK), a member of the SNF1 (sucrose non fermentor) kinase family, is a heterotrimeric protein comprise of alpha (63 kDa), beta (30 kDa) and gamma (38 kDa) subunits. The alpha subunit is the catalytic subunit, while beta and gamma are non catalytic subunits (although they have been found to interact with the active subunit in liver). AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase. AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro.Synonyms: 5'-AMP-activated protein kinase subunit gamma-1/2/3, AMPK gamma-1, AMPK gamma-2, AMPK gamma-3, AMPKG3, AMPKg, H91620p, PRKAG1, PRKAG2, PRKAG3

Molekulargewicht: approx. 40,42 kDa

Gen-ID: 5571

NCBI Accession: NP_002724

UniProt: P54619

Pathways: AMPK Signaling, Regulation of Carbohydrate Metabolic Process, Warburg Effekt