TNFSF9 Antikörper (Biotin)

Produktnummer ABIN465601

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch TNFSF9 in WB und ELISA. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für TNFSF9 Antikörper (Biotin) (ABIN465601)

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TNFSF9 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-TNFSF9 Antikörper (Biotin)

Spezifität: Human 4-1BBL

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human 4-1BBL.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect h4-1BBL by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant h4-1BBL. To detect h4-1BBL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human 4-1BBL (LS-B7425) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant h4-1BBL. Western Blot: To detect h4-1BBL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant h4-1BBL is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS containing 0.1 % BSA

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu TNFSF9

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Andere Bezeichnung: TNFSF9 / CD137L

Hintergrund: Name/Gene ID: TNFSF9
Family: TNF

Synonyms: TNFSF9, 4-1BBL, 4-1BB ligand, 4-1BB-L, CD137L, Homolog of mouse 4-1BB-L, Receptor 4-1BB ligand

Gen-ID: 8744

UniProt: P41273

Pathways: Activated T Cell Proliferation, Cancer Immune Checkpoints