Signal Transducer and Activator of Transcription 3 (STAT3) (S727) Antikörper

Antigen: Signal Transducer and Activator of Transcription 3 (STAT3)

Synonyme: APRF, HIES, FLJ20882, MGC16063, Aprf, AW109958, 1110034C02Rik, MGC93551, MGC128731, STAT3, stat3

Epitope: S727

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (IHC), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN389664

Produktbeschreibung

Produktmerkmale: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: Phospho-STAT3-S727

Gen-ID: 6774

Swiss-Prot: P40763

Immunogen: This Phospho-STAT3-S727 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S727 of human STAT3.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: Signal transducer and activator of transcription 3, Acute-phase response factor, STAT3, APRF,

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S727 of human STAT3.

Molekulargewicht: 88068 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylate

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Signaltransduktion, Phospho-Antikörper, Zelluläre Signaltransduktion, Proteinmodifikationen, Transkriptionsfaktoren, Neurologie, Zellstruktur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

The Phospho-STAT3-S727 Pab (3260a) is used in western blot to detect Ser727-phosphorylated STAT3 in mouse liver tissue lysates collected before (-) or after (+) stimulation obtained by IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute (La Jolla, CA).

The phospho-STAT3-Ser727 Pab (ABIN389664) is used in Western blot to detect Ser727-phosphorylated STAT3 in HL-60 tissue lysates collected before (-) or after (+) stimulation obtained by IFN-a.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.