LC3 (APG8B) (N-term) ¨C 22 citations Antikörper

Antigen: LC3 (APG8B) (N-term) ¨C 22 citations

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Ratte (Rattus)

Applikation: Western Blot (WB), Immunfluoreszenz (IF), Immunhistochemie (IHC), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN388473

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Gen-ID: 81631

Swiss-Prot: Q9GZQ8

Immunogen: This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human LC3 (APG8b).

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: MAP1LC3B, LC3B, MAP1A/1BLC3, MAP1LC3B

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the N-terminal region of human LC3 (APG8b).

Molekulargewicht: 14557 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Immunofluorescence~~1:50~~100

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Forschungsgebiet: Zelluläre Signaltransduktion, Zellstruktur, Autophagie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Left: Western blot analysis of anti-LC3 (APG8b) Pab (Cat. #ABIN388473) in rat brain lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in membrane fraction (P) but pro-LC3 (APG8b) and non-lipidated LC3 ((APG8b) were detected in soluble fraction (S). Right: Western blot analysis of anti-LC3 (APG8b) Pab (Cat. #ABIN388473) in 293 cell line lysates transiently transfected with the LC3 (APG8b) gene (2ug/lane). Human LC3 (APG8b) (arrow) was detected using the purified Pab (1:60 dilution). TOP: FACS sorted LSK cells (Lineage-Sca-1+c-kit+, enriched for hematopoietic stem cells) incubated with LC3 antibody (Cat.# ABIN388473) at 1:50 dilution at 4 degree overnight., and detected with AlexaFlour 488 conjugated anti-rabbit antibody (1:200). Data courtesy of Wenbin Xiao, La Jolla Institute for Allergy and Immunology. CENTER: Figure 1 - Immunofluorescence analysis of anti-LC3 (APG8b) polyclonal antibody in rat brain before (left) and after (right) treatment with rapamycin (100 nm for 4 hr). CENTER: Figure 2 - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated. BC = breast carcinoma, HC = hepatocarcinoma. BOTTOM: Figure 1 - Wild-type (Cln3+/+) or homozygous Cln3Äex7/8 (Cln3Äex7/8/Äex7/8) paraffin-embedded brain sections immunostained for the LC3 protein (Cat. # ABIN388473 LC3 antibody). Shown are the CA2/CA3 region of hippocampus (Hc) and cerebellum (Cb) from 10-month-old mice. Few immunopositive puncta are present in wild-type sections, whereas homozygous Cln3Äex7/8 sections contain clusters of LC3-positive puncta around pyramidal neurons and Purkinje cells (P). MOL, molecular layer, GCL, granule cell layer. Data courtesy of Dr. Susan Cotman, Massachusets General Hospital. BOTTOM: Figure 2 - Immunoblots for LC3 protein (Cat. # ABIN388473 LC3 antibody) are shown for extracts from untreated or rapamycin-treated (250 nM, 4 h) cultures of wild-type (CbCln3+/+) or homozygous cerebellar cells (CbCln3Äex7/8/Äex7/8). Rapamycin increases the LC3-II/LC3-I ratio in wild-type and homogyzous cerebellar cell extracts (2.6 and 2.2-fold, respectively), consistent with activation of autophagy.

anti-LC3 (APG8B) (N-term) ¨C 22 citations antibody (Image 2)

Publikationen

Publikationen: English, Chemali, Duron et al.: "Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection." in: Nature immunology, Vol. 10, Issue 5, pp. 480-7, 2009 (PubMed).

Viiri, Hyttinen, Ryhaenen et al.: "p62/sequestosome 1 as a regulator of proteasome inhibitor-induced autophagy in human retinal pigment epithelial cells." in: Molecular vision, Vol. 16, pp. 1399-414, 2010 (PubMed).