XBP1 Antikörper (Internal Region)

Produktnummer ABIN374982

Zitiert in 1 Publikation. Der Ziege Polyklonal anti-XBP1 Antikörper (ABIN374982) detektiert spezifisch XBP1 in WB, IHC (p) und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Hund.

Kurzübersicht für XBP1 Antikörper (Internal Region) (ABIN374982)

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Reaktivität: Human, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser XBP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-XBP1 Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-NHSWEDTFAN ELFPQ

Spezifität: This antibody is expected to recognize isoform XBP1(S) only.

Kreuzreaktivität (Details): Species reactivity (expected):Canine.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Synthetic peptide from an Internal region of Human XBP1 (NP_001073007.1) Genename: XBP1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection Limit Dilution: 1/4000. Western Blot: 1-3 μg/mL. Approx 38 kDa band observed in Nuclear lysates of cell line HeLa(calculated MW of 40.8 kDa according to NP_001073007.1). Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-XBP1 Antikörper (ABIN374982)

Yokouchi, Hiramatsu, Hayakawa, Okamura, Du, Kasai, Takano, Shitamura, Shimada, Yao, Kitamura: "Involvement of selective reactive oxygen species upstream of proapoptotic branches of unfolded protein response." in: The Journal of biological chemistry, Vol. 283, Issue 7, pp. 4252-60, (2008) (PubMed).

Details zu XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Andere Bezeichnung: XBP1 / TREB5

Hintergrund: This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. The protein contains two domains commonly found in transcription factors that confer DNA binding and dimerization capability. This gene product was also separately identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. There is also a recent finding that the same agents that cause an ER stress response, the unfolded protein response, also increase transcription of this gene. This increase in transcription requires an ER stress response consensus binding element in the promoter.Synonyms: Tax-responsive element-binding protein 5, X-box-binding protein 1, XBP-1, XBP2

Gen-ID: 7494, 9606

UniProt: P17861

Pathways: ER-Nucleus Signaling, Unfolded Protein Response