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Antikörper
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anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antikörper
Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antikörper
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| Synonyme |
KIP1, MEN4, CDKN4, MEN1B, P27KIP1, p27, Rpn4, MGC8644, Kip1, Cdki1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, CDKN1B |
| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Applikation
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| Produktnummer |
ABIN374656 |
| Menge |
0.1 mg (0.5 mg/ml) (Varianten) |
| Preis |
250,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Produktmerkmale
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Synonyms: p27Kip1, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27
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Weitere Bezeichnung
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CDKN1B / KIP1
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Gen-ID
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1027
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Swiss-Prot
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P46527
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Immunogen
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Peptide with sequence C-KRPATDDSSTQNK, from the internal region of the proteinsequence
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Kreuzreaktivität
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Hund, Katze, Human
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Beschreibung
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Cell cycle progression is regulated by cyclins and their cognate Cdks. p27 KIP 1 is a cellcycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressorgene, and has been proposed to function as a possible mediator of TGF beta induced G1arrest. p27 KIP 1 is up regulated in response to antimitogenic stimuli. The increased proteinexpression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such ascyclin D1/Cdk4. p27 Kip1 is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27 Kip 1,while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.
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Spezifität
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This antibody reacts to CDKN1B. Species Reactivity: Tested: Human. Expected from sequence similarity: Feline, Canine.
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Applikationshinweise
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Peptide ELISA: 1/32000. Western Blot: 0.1 - 0.3 µg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Konzentration
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0.5 mg/ml
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Reinigung
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Aff - Purified
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Buffer
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Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
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Lagerung
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Forschungsgebiet
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Krebs, Zellzyklus
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B)", Typ "Antikörper" finden
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Wirte
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Kaninchen (61), Maus (20), Ziege (7), Huhn (2)
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Reaktivitäten
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Human (81), Maus (36), Ratte (Rattus) (32), Hund (4), Affe (2), Huhn (1), Rind (Kuh) (1)
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Applikationen
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Western Blot (WB) (75), Immunhistochemie (Paraffinschnitte) (IHC (p)) (38), Immunpräzipitation (IP) (19), Enzyme Immunoassay (EIA) (18), ELISA (10), Immunfluoreszenz (IF) (10), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (9), Immunhistochemie (IHC) (9), ELISA (Detektionsantikörper) (5), Dot Blot (Dot) (3)
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Epitope
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pThr187 (5), internal (2), pSer178 (2), pThr157 (2), pThr198 (2), C-Term (1), T198 (1), pS10 (1), pS178 (1), pSer10 (1), pT157 (1), pT187 (1)
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Alternativen 
