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Antikörper
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anti-Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2) Antikörper
Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2) Antikörper
| Antigen |
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| Synonyme |
CD, ACUG, BLAU, IBD1, NLRC2, NOD2B, CARD15, CLR16.3, PSORAS1 |
| Klonalität |
Monoklonal (2D9) |
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Wirt
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Reaktivität
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Applikation
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 Alternativen Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunpräzipitation (IP), Western Blot (WB)
Western Blot (WB) (38), Immunfluoreszenz (IF) (23), Durchflusszytometrie (FACS) (17), Immunhistochemie (Paraffinschnitte) (IHC (p)) (15), Immunpräzipitation (IP) (11), Enzyme Immunoassay (EIA) (6), ELISA (5), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (4), Dot Blot (Dot) (1), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (1)
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| Produktnummer |
ABIN363229 |
| Menge |
0,1 ml (2,25 mg/ml) |
| Preis |
350,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 3 bis 5 Werktagen |
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Immunogen
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Recombinant human NOD2 protein corresponding to residues 28-301
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Format
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Protein G purified
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Isotyp
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IgG1 (Passende Sekundärantikörper)
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Klon
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2D9
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Spezifität
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Monoclonal antibody reacts specifically with NOD2 protein. Cross Reactivities: This antibody reacts with human protein. Other species have not been tested
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Applikationshinweise
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This antibody has been tested for Western Blot of NOD2 transfected 293T lysates and HT29 human colorectal adenocarcinoma lysates (endogenous detection has some background) where a band is seen at ~110 kDa. Other applications have not been tested. Working Dilutions: Optimal dilution should be determined by the end user The following are guidelines only : - Immunohistochemistry (paraffin) : 1/100 to 1/1 000 - ipon : 1/100 to 1/1 000 - Western Blot : 1/100 to 1/1 000
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Konzentration
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2,25 mg/ml
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Buffer
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Constituents: Tris-glycine buffer containing 150mM NaCl. Preservative: NaN3
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Lagerung
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Short term storage : +4°C Long term storage : -20°C Avoid freeze-thaw cycles
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Publikationen
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Cooney, Baker, Brain et al.: "NOD2 stimulation induces autophagy in dendritic cells influencing bacterial handling and antigen presentation." in: Nature medicine, Vol. 16, Issue 1, pp. 90-7, 2010 (PubMed).
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Alternativen zu Antigen "Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2)", Typ "Antikörper" finden
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Wirte
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Kaninchen (42), Maus (8), Ziege (5), Ratte (1)
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Reaktivitäten
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Human (53), Maus (14), Ratte (Rattus) (10), Hund (4), Affe (4), Schwein (3), Rind (Kuh) (2), Kaninchen (2), Huhn (1), Zebrafisch (Brachydanio rerio) (1)
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Applikationen
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Western Blot (WB) (38), Immunfluoreszenz (IF) (23), Durchflusszytometrie (FACS) (17), Immunhistochemie (Paraffinschnitte) (IHC (p)) (15), Immunpräzipitation (IP) (11), Enzyme Immunoassay (EIA) (6), ELISA (5), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (4), Dot Blot (Dot) (1), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (1)
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Konjugate
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Biotin (3), HRP (3), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitope
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C-Term (3), internal (2), AA 125-140 (1), N-Term (1)
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