Polo-Like Kinase 1 (PLK1) (pThr210) Antikörper

Antigen: Polo-Like Kinase 1 (PLK1)

Synonyme: PLK, STPK13, Snk, plk, MGC52566, Plx1, PLK-1, stpk13, MGC69251, MGC133927

Epitope: pThr210

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Ratte (Rattus), Human

Applikation: Western Blot (WB)

Produktnummer: ABIN361457

Produktbeschreibung

Weitere Bezeichnung: Phospho-Thr210 Polo-like Kinase 1

Format: Affinity-purified

Beschreibung: Polo-like kinases are important regulators of cell cycle progression. PLK1 is a highly conserved Ser/Thr kinase that has essential roles in the formation of mitotic bipolar spindles (van Vugt et al., 2004). Deregulated expression of PLK?s is detected in many types of cancer and associated with oncogenesis (Takei et al., 2005). It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser137 and Thr210 (van de Weerdt et al., 2005). Anti-Phospho-Thr210 PLK1 Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 k PLK protein phosphorylated at Thr210 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr210 PLK antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Spezifität: Specific for ~66k PLK phosphorylated at Thr210. Immunolabeling of the PLK band is completely blocked by (-phosphatase treatment.

Anwendungen

Applikationshinweise: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Reinigung: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Lagerung: Store at -20 °C, stable for 1 year

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blots of rat synaptic membrane showing specific immunolabeling of the ~66 k PLK protein phosphorylated at Thr210 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr210 PLK antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.