STAT1 Antikörper (pSer727)

Produktnummer ABIN358231

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch STAT1 in WB, IHC (p) und EIA. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für STAT1 Antikörper (pSer727) (ABIN358231)

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser STAT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-STAT1 Antikörper

Bindungsspezifität: pSer727

Spezifität: This antibody detects STAT1 pSer727. Predicted to cross react with Mouse (100 % Antigen Homology).

Aufreinigung: Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S727 of human STAT1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu STAT1

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Andere Bezeichnung: STAT1

Hintergrund: In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens.Synonyms: Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84

Molekulargewicht: 87335 Da

Gen-ID: 6772, 9606

UniProt: P42224

Pathways: JAK-STAT Signalweg, RTK Signalweg, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events