GLI2 Antikörper (AA 46-60)

Produktnummer ABIN351530

Der Kaninchen Polyklonal anti-GLI2 Antikörper wird verwendet zum Nachweis von GLI2 in Proben von Human. Er wurde validiert für WB, ELISA, IHC und IHC (p).

Kurzübersicht für GLI2 Antikörper (AA 46-60) (ABIN351530)

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLI2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GLI2 Antikörper

Bindungsspezifität: AA 46-60

Spezifität: Amino acids 46-60 of human Gli-2 (isoform a).

Aufreinigung: Immunoaffinity purified

Immunogen: Corresponds to amino acids 46-60 of human Gli-2 (isoform a).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:2000 - 1:12000), IHC, IHC-P (5 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Multiple splice variants have been reported for this protein. Detection of Gli-2 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu GLI2

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Andere Bezeichnung: GLI2

Hintergrund: Name/Gene ID: GLI2

Synonyms: GLI2, GLI-Kruppel family member GLI2, HPE9, Gli-2, Oncogene GLI2, Tax helper protein 2, THP, Zinc finger protein GLI2, Tax helper protein, THP2, GLI family zinc finger 2, Tax helper protein 1, THP1

Gen-ID: 2736

UniProt: P10070

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis