ARPC2 Antikörper (AA 288-300)

Produktnummer ABIN351464

Der Ziege Polyklonal anti-ARPC2 Antikörper wird verwendet zum Nachweis von ARPC2 in Proben von Human, Maus, Ratte, Rind (Kuh), Kaninchen, Hund, Hamster und Affe. Er wurde validiert für WB, ELISA, IHC und IHC (p).

Kurzübersicht für ARPC2 Antikörper (AA 288-300) (ABIN351464)

Target: ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen, Hund, Hamster, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ARPC2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ARPC2 Antikörper

Bindungsspezifität: AA 288-300

Spezifität: Human ARPC2. The reported variants NP_005722.1 and NP_690601.1 represent identical protein.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Rabbit, Opossum, Platypus (100%) Horse, Lizard (92%) Turkey, Chicken, Xenopus (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-EMKTITGKTFSSR from the C-terminus of human ARPC2 (NP_005722.1, NP_690601.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Rabbit, Opossum, Platypus (100%), Horse, Lizard (92%), Turkey, Chicken, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (0.1 - 0.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu ARPC2

Target: ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))

Andere Bezeichnung: ARPC2 / p34-Arc

Hintergrund: Name/Gene ID: ARPC2
Family: Cytoskeleton

Synonyms: ARPC2, Arp2/3 complex 34 kDa subunit, p34-Arc, PNAS-139, PRO2446, ARC34

Gen-ID: 10109

NCBI Accession: NP_005722, NP_690601

UniProt: O15144

Pathways: RTK Signalweg, Regulation of Actin Filament Polymerization