STAT3 Antikörper (Internal Region)

Produktnummer ABIN185692

Dieser Ziege Polyklonal Antikörper detektiert spezifisch STAT3 in WB, IHC, ELISA und FACS. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für STAT3 Antikörper (Internal Region) (ABIN185692)

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser STAT3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-STAT3 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: STAT3

Sequenz: CRPESQEHPE ADP

Spezifität: This antibody is expected to recognise all three reported isoforms (NP_644805.1, NP_003141.2 and NP_998827.1).

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CRPESQEHPEADP, from the internal region of the protein sequence according to NP_644805.1, NP_003141.2, NP_998827.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 85 kDa band observed in lysates of cell line LNCaP, and approx. 90 kDa in Mouse Heart lysates (calculated MW of 83.1 kDa according to Human NP_998827.1 and 88.1 kDa according to Mouse : NP_998824.1). Recommended concentration: 0.5-3 μg/mL

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu STAT3

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Andere Bezeichnung: STAT3

Hintergrund: STAT3, signal transducer and activator of transcription 3 (acute-phase response factor), APRF, FLJ20882, MGC16063 , DNA-binding protein APRF, acute-phase response factor, signal transducer and activator of transcription 3

Gen-ID: 6774, 20848, 25125

NCBI Accession: NP_644805, NP_003141, NP_998827

Pathways: JAK-STAT Signalweg, RTK Signalweg, Interferon-gamma Pathway, Neurotrophin Signalübertragung, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor