Cyclin D1 (CCND1) Antikörper

Antigen: Cyclin D1 (CCND1)

Synonyme: BCL1, PRAD1, U21B31, D11S287E, cD1, Cyl-1, bcl-1, AI327039, DIP1, GCIP, Maid, SECC-8, SSEC-8, AU022347, cb161, cycd1, fb52e01, fc83a12, id:ibd1198, wu:fb52e01, wu:fc83a12, MGC109078, ccnd1b, MGC52909, MGC132138, ccnd1, MGC53090, bcl1, prad1, u21b31, MGC69515, d11s287e, CCND1, ccndbp1, MGC137744, ZHM, CCNDBP1, MGC133757, DKFZp468E2216, DKFZp469C1216, DKFZp469H0317

Klonalität: Monoklonal (CD1-1)

Wirt: Maus

Reaktivität: Human, Ratte (Rattus)

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Western Blot (WB), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunhistochemie (Paraffinschnitte) (IHC (p)), ELISA, Immunzytochemie (ICC)

Produktnummer: ABIN302101

Produktbeschreibung

Weitere Bezeichnung: Cyclin D1

Immunogen: Purified cyclin D1 protein

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: CD1-1

Beschreibung: Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized duringG1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 inresponse to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate thegrowth-suppressive function of the Rb protein through its phosphorylation, and titrate CDKinhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulatesin the nucleus, it translocates into the cytoplasm during S phase. Without growthfactor-mediated stimulation cyclin D1 is unstable, and undergoes ubiquitin-mediateddegradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participatesin G1/S phase arrest.

Spezifität: The antibody CD1.1 recognizes cyclin D1, an ubiquitously expressed 33 kDa protein thatmigrates as a 36 kDa band under reducing SDS-PAGE conditions.

Synonyme: BCL1, PRAD1, U21B31, D11S287E, cD1, Cyl-1, bcl-1, AI327039, DIP1, GCIP, Maid, SECC-8, SSEC-8, AU022347, cb161, cycd1, fb52e01, fc83a12, id:ibd1198, wu:fb52e01, wu:fc83a12, MGC109078, ccnd1b, MGC52909, MGC132138, ccnd1, MGC53090, bcl1, prad1, u21b31, MGC69515, d11s287e, CCND1, ccndbp1, MGC137744, ZHM, CCNDBP1, MGC133757, DKFZp468E2216, DKFZp469C1216, DKFZp469H0317

Anwendungen

Applikationshinweise: Flow Cytometry. Application note: Membrane permeabilization is required. Immunoprecipitation - Recommended dilution: 1 µg/ml. - Recommended dilution: 1 µg/ml. Immunohistochemistry (paraffin sections) - Pretreatment: Heat treatment, sodium citrate buffer pH 6. 0.Immunohistochemistry (frozen sections) - Recommended dilution: 2 µg/ml. Positive tissue: colon. Immunocytochemistry - Recommended dilution: 1 µg/ml

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Krebs, Zellzyklus

Beschränkungen: Nur für Forschungszwecke einsetzbar