SMAD4 Antikörper

Produktnummer ABIN272224

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch SMAD4 in WB, IF und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte. Es wurde in 6+ Publikationen zitiert.

Kurzübersicht für SMAD4 Antikörper (ABIN272224)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SMAD4 Antikörper

Spezifität: This antibody detects endogenous levels of Smad4 protein. (region surrounding Leu43)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Referenzen für anti-SMAD4 Antikörper (ABIN272224)

Nagappan, Park, Park, Kim, Hong, Kang, Zhang, Kim, Lee, Won, Kim: "Proteomic analysis of differentially expressed proteins in vitamin C-treated AGS cells." in: BMC biochemistry, Vol. 14, pp. 24, (2014) (PubMed).

Yang, Zhao, Rasul, Qin, Li, Li: "PIAS1-modulated Smad2/4 complex activation is involved in zinc-induced cancer cell apoptosis." in: Cell death & disease, Vol. 4, pp. e811, (2013) (PubMed).

Yang, Li, Wang, Wang, Li, Yang, Peng: "Smad4 disruption accelerates keratinocyte reepithelialization in murine cutaneous wound repair." in: Histochemistry and cell biology, Vol. 138, Issue 4, pp. 573-82, (2012) (PubMed).

Yan, Zhang, Fang, Zhang, Wu, Jiang, Sun, Hu: "MicroRNA-145 suppresses mouse granulosa cell proliferation by targeting activin receptor IB." in: FEBS letters, Vol. 586, Issue 19, pp. 3263-70, (2012) (PubMed).

Izumi, Nakamura, Tokumo, Mano: "A minute pancreatic ductal adenocarcinoma with lipomatous pseudohypertrophy of the pancreas." in: JOP : Journal of the pancreas, Vol. 12, Issue 5, pp. 464-8, (2011) (PubMed).

Yuan, Wang, Hu, Li, Jing: "Transforming growth factor-?/Smad signaling function in the aortopathies." in: Revista brasileira de cirurgia cardiovascular : órgão oficial da Sociedade Brasileira de Cirurgia Cardiovascular, Vol. 26, Issue 3, pp. 393-403, (2011) (PubMed).

Details zu SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Andere Bezeichnung: SMAD4

Hintergrund: Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.Synonyms: DPC4, Deletion target in pancreatic carcinoma 4, MAD homolog 4, MADH4, Mothers against DPP homolog 4, Mothers against decapentaplegic homolog 4, SMAD 4, SMAD family member 4, SMAD-4

Molekulargewicht: approx. 60 kDa

Gen-ID: 4089

NCBI Accession: NP_005350

UniProt: Q13485

Pathways: Zellzyklus, Chromatin Binding, Autophagie