Exonuclease 1 (EXO1) Antikörper

Antigen: Exonuclease 1 (EXO1)

Synonyme: ATWEX, ATWRNEXO, EXONUCLEASE, F18A5.260, F18A5_260, WERNER SYNDROME-LIKE EXONUCLEASE, WEX, WRNEXO, HEX1, hExoI, Msa, 5730442G03Rik, EXO1

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN238661

Produktbeschreibung

Weitere Bezeichnung: EXO1

Gen-ID: 9156

Immunogen: C-HRNYSPRPESGT

Format: Affinity-purified

Beschreibung: All names and symbols: EXO1, exonuclease 1, HEX1, hExoI, rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1. Official Symbol: EXO1 . RefSeq number(s): NP_003677.3, NP_006018.3, NP_569082.1

Spezifität: This antibody is expected to recognise all three reported isoforms (NP_003677.3, NP_006018.3 and NP_569082.1) Human

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 90kDa band observed in lysates in cell lines HEK293 and A549 (calculated MW of 89.1kDa according to NP_003677.3). Recommended concentration: 0.2-0.5µg/ml. An additional band of unknown identity was also consistently observed at 38kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Metabolismus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN238661 (0.2µg/ml) staining of A549 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Kim, Roh, Yoon et al.: "MLH3 and EXO1 alterations in familial colorectal cancer patients not fulfilling Amsterdam criteria." in: Cancer genetics and cytogenetics, Vol. 176, Issue 2, pp. 172-4, 2007 (PubMed).