STAT1 Antikörper (pTyr701)

Produktnummer ABIN233804

Hersteller Produkt- Nr.: 200-401-a43

Der Kaninchen Polyklonal anti-STAT1 Antikörper wird verwendet zum Nachweis von STAT1 in Proben von Human. Er wurde validiert für WB und ELISA.

Kurzübersicht für STAT1 Antikörper (pTyr701) (ABIN233804)

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser STAT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-STAT1 Antikörper

Bindungsspezifität: pTyr701

Hersteller Produkt- Nr.: 200-401-a43

Hersteller: Rockland

Verwendungszweck: Stat1 phospho Y701 Antibody

Kreuzreaktivität (Details): This antibody is specific for mouse Stat1 protein phosphorylated at Y701.

Produktmerkmale: Synonyms: rabbit anti-Stat1 pY701 Antibody, DKFZp686B04100 antibody, ISGF 3 antibody, Signal transducer and activator of transcription 1 91 kDa antibody, Signal transducer and activator of transcription 1 alpha/beta antibody, Signal transducer and activator of transcription 1 antibody

Aufreinigung: This product was Protein A purified from monospecific antiserum.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y701 in the mouse STAT1 protein.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat1 protein by western blotting in the appropriate cell lysate or extract.  This phospho-specific polyclonal antibody reacts with mouse Stat1 pY701 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:25,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 2.3 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu STAT1

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Andere Bezeichnung: Stat1

Hintergrund: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). Signal transducer and activator of transcription 1 (Stat1) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by cell surface receptors IFN (interferon), cytokine KITLG/SCF and other cytokines and other growth factors. Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation and DNA binding. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Anti-Stat1pY701 Antibody is useful for researchers interested in transcription factor, cytokines and growth factor, Interleukin, Interferon gamma signaling, Cancer, Immunology, and Nuclear Signaling research.

Gen-ID: 20846, 114326482

UniProt: Q99K94

Pathways: JAK-STAT Signalweg, RTK Signalweg, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events