GLI2 Antikörper (Internal Region)

Produktnummer ABIN214612

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch GLI2 in WB, ELISA, IHC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für GLI2 Antikörper (Internal Region) (ABIN214612)

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLI2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GLI2 Antikörper

Bindungsspezifität: Internal Region

Spezifität: Amino acids from an internal region of Mouse Gli-2.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from mouse GLI2.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:15000 - 1:60000), IHC, IHC-P (5 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu GLI2

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Andere Bezeichnung: GLI2

Hintergrund: Name/Gene ID: GLI2

Synonyms: GLI2, GLI-Kruppel family member GLI2, HPE9, Gli-2, Oncogene GLI2, Tax helper protein 2, THP, Zinc finger protein GLI2, Tax helper protein, THP2, GLI family zinc finger 2, Tax helper protein 1, THP1

Gen-ID: 2736

UniProt: P10070

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis