X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6) Antikörper

Antigen: X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6)

Synonyme: ML8, KU70, TLAA, CTC75, CTCBF, G22P1, XRCC6, MGC82304, DKFZp459H0114

Klonalität: Monoklonal (N3H10)

Wirt: Maus

Reaktivität: Human, Affe, Xenopus laevis

Applikation: Immunfluoreszenz (IF), Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN187448

Produktbeschreibung

Weitere Bezeichnung: XRCC6 / Ku70

Immunogen: Human placental extract designated as PSE1-PL.

Isotyp: IgG2b  (Passende Sekundärantikörper)

Klon: N3H10

Beschreibung: The Ku autoantigen is a heterodimer of 70 kDa (p70) and ~80 kDa (p80) proteins. The p70/p80 dimer is important for the function of a 460 kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53 and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation and apoptosis.

Spezifität: Reacts with the p70 Ku antigen subunit. Recognizes an epitope between aa 506-541 of p70. Species Reactivity: Human, monkey and Xenopus. Mouse and hamster (Western blots only). Does not react with bovine, rabbit or sea urchin. Other species not tested. Cellular Location: Nuclear.Positive Control: BT474, T47D, HEP-G-2 or HeLa cells. Tonsil.

Anwendungen

Applikationshinweise: Immunofluorescence Immunohistochemistry: (Formalin/paraffin) Use at a 1:400 dilution for 30 minutes at room temperature (RT). Staining of formalin-fixed tissues is improved by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling to RT for 20 min. Immunoprecipitation: (Native and denatured) Use at 2 µg/mg protein lysate. Use Protein A. Western blot: Use at 0.25-0.5 µg/mL for 2 hrs. at RT. The optimal dilution for a specific application should be determined by the researcher.

Buffer: 100 µg of monoclonal antibody at 200 µg/mL in 10 mM PBS, pH 7.4, with protein stabilizer and 0.09% sodium azide. Purified from ascites fluid by Protein A chromatography.

Lagerung: Store at 4 °C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6) antibody

anti-X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6) antibody (Image 2)

Publikationen

Publikationen: Shi, Qiu, Zhao et al.: "Dynamic binding of Ku80, Ku70 and NF90 to the IL-2 promoter in vivo in activated T-cells." in: Nucleic acids research, Vol. 35, Issue 7, pp. 2302-10, 2007 (PubMed).

Wu, Frit, Malivert et al.: "Interplay between Cernunnos-XLF and nonhomologous end-joining proteins at DNA ends in the cell." in: The Journal of biological chemistry, Vol. 282, Issue 44, pp. 31937-43, 2007 (PubMed).

Cheung, Salerno, Hanakahi: "Evidence for an inositol hexakisphosphate-dependent role for Ku in mammalian nonhomologous end joining that is independent of its role in the DNA-dependent protein kinase." in: Nucleic acids research, Vol. 36, Issue 17, pp. 5713-26, 2008 (PubMed).

Meulle, Salles, Daviaud et al.: "Positive regulation of DNA double strand break repair activity during differentiation of long life span cells: the example of adipogenesis." in: PLoS ONE, Vol. 3, Issue 10, pp. e3345, 2008 (PubMed).

Gama, Gomez, Mayo et al.: "Hdm2 is a ubiquitin ligase of Ku70-Akt promotes cell survival by inhibiting Hdm2-dependent Ku70 destabilization." in: Cell death and differentiation, Vol. 16, Issue 5, pp. 758-69, 2009 (PubMed).

Yu, Sekine-Suzuki, Xue et al.: "Chemopreventive agent sulforaphane enhances radiosensitivity in human tumor cells." in: International journal of cancer. Journal international du cancer, Vol. 125, Issue 5, pp. 1205-11, 2009 (PubMed).