CD14 Molecule (CD14) Antikörper

Antigen: CD14 Molecule (CD14)

Synonyme: CD14

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN185563

Produktbeschreibung

Weitere Bezeichnung: CD14

Gen-ID: 929

Immunogen: C-KRVDADADPRQYAD

Format: Affinity-purified

Beschreibung: All names and symbols: CD14, CD14 antigen , HGNC:1628. Official Symbol: CD14. RefSeq number(s): NP_000582.1

Spezifität: Human 375 aa

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:128,000. Approx 55kDa band observed in Human Lung, Lymph Node and Tonsil lysates (calculated MW of 40.1kDa according to NP_000582.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun. 2001 Jun,69(6):3772-81, PMID: 11349042). Recommended concentration: 1-3µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Stammzellen, Angeborene Immunität, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, CD Antigene, Oberflächenrezeptoren der Immunzellen , Apoptose/Nekrose, Immunologie, Inflammation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185563 (1µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Kim, Lee, Jin et al.: "Crystal structure of CD14 and its implications for lipopolysaccharide signaling." in: The Journal of biological chemistry, Vol. 280, Issue 12, pp. 11347-51, 2005 (PubMed).

Iwaki, Nishitani, Mitsuzawa et al.: "The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain." in: Biochemical and biophysical research communications, Vol. 328, Issue 1, pp. 173-6, 2005 (PubMed).