SDHB Antikörper (C-Term)

Produktnummer ABIN185447

Der Ziege Polyklonal anti-SDHB Antikörper wird verwendet zum Nachweis von SDHB in Proben von Human. Er wurde validiert für WB, IHC, ELISA, FACS und IF.

Kurzübersicht für SDHB Antikörper (C-Term) (ABIN185447)

Target: SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SDHB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-SDHB Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: SDHB

Sequenz: ATYKEKKASV

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ATYKEKKASV, from the C Terminus of the protein sequence according to NP_002991.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 6-8 μg/mL.

Western Blot: Approx 28-30 kDa band observed in lysates of cell lines HEK293, Jurkat and HeLa (calculated MW of 31.6 kDa according to NP_002991.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:8000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the plasm membrane/ cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentr

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu SDHB

Target: SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))

Andere Bezeichnung: SDHB

Hintergrund: SDHB, IP, SDH, SDH1, SDHIP, succinate dehydrogenase complex, subunit B, iron sulfur (Ip), OTTHUMP00000044624, iron-sulfur subunit, FLJ92337, PGL4, SDH2, SDHIP

Gen-ID: 6390

NCBI Accession: NP_002991