ARPC2 Antikörper (C-Term)

Produktnummer ABIN184748

Es gibt 1 Publikation für dieses Produkt. Der Ziege Polyklonal anti-ARPC2 Antikörper wird verwendet zum Nachweis von ARPC2 in Proben von Human. Er wurde validiert für WB, ELISA, IHC und IF.

Kurzübersicht für ARPC2 Antikörper (C-Term) (ABIN184748)

Target: ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ARPC2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-ARPC2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: ARPC2

Sequenz: EMKTITGKTF SSR

Spezifität: The reported variants NP_005722.1 and NP_690601.1 represent identical protein.

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EMKTITGKTFSSR, from the C Terminus of the protein sequence according to NP_005722.1, NP_690601.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 34-35 kDa band observed in lysates of cell lines CACO-2, HeLa and Jurkat (calculated MW of 34.3 kDa according to NP_005722.1). Recommended concentration: 0.1-0.5 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-ARPC2 Antikörper (ABIN184748)

Sakata, Taniguchi, Yasuda, Adachi-Morishima, Hamazaki, Nakayama, Miki, Minato, Narumiya: "Impaired T lymphocyte trafficking in mice deficient in an actin-nucleating protein, mDia1." in: The Journal of experimental medicine, Vol. 204, Issue 9, pp. 2031-8, (2007) (PubMed).

Details zu ARPC2

Target: ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))

Andere Bezeichnung: ARPC2

Hintergrund: ARPC2, actin related protein 2/3 complex, subunit 2, 34 kDa, ARC34, PRO2446, p34-Arc, PNAS-139, ARP2/3 protein compex subunit 34, actin related protein 2/3 complex subunit 2

Gen-ID: 10109, 76709

NCBI Accession: NP_005722, NP_690601

Pathways: RTK Signalweg, Regulation of Actin Filament Polymerization