STAT3 Antikörper (C-Term)

Produktnummer ABIN184593

Der Ziege Polyklonal anti-STAT3 Antikörper (ABIN184593) detektiert spezifisch STAT3 in IHC, ELISA, IF und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für STAT3 Antikörper (C-Term) (ABIN184593)

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser STAT3 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-STAT3 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: STAT3 (isoform 1 and 2)

Sequenz: DMELTSECAT SPM

Spezifität: This antibody is expected to recognise isoforms 1 and 2 (as represented by NP_644805.1 and NP_003141.2 respectively)

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence DMELTSECATSPM, from the C Terminus of the protein sequence according to NP_003141.2, NP_644805.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Mouse Thymus shows preferential staining of medula over cortex. Recommended concentration: 4-6 μg/mL.

Western Blot: Preliminary testing showed a band at approx 80-85 kDa in A431 nuclear cell lysate after 2 μg/mL antibody staining (calculated MW of 80 kDa according to NP_003141.2, and NP_644805.1). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:8000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu STAT3

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Andere Bezeichnung: STAT3

Hintergrund: STAT3, signal transducer and activator of transcription 3 (acute-phase response factor), APRF, DNA-binding protein APRF, acute-phase response factor, FLJ20882, MGC16063, HIES

Gen-ID: 6774, 20848, 25125

NCBI Accession: NP_003141, NP_644805

Pathways: JAK-STAT Signalweg, RTK Signalweg, Interferon-gamma Pathway, Neurotrophin Signalübertragung, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor