Cyclin-Dependent Kinase Inhibitor 1B (CDKN1B) (C-Term) Antikörper

Antigen: Cyclin-Dependent Kinase Inhibitor 1B (CDKN1B)

Synonyme: KIP1, MEN4, CDKN4, MEN1B, P27KIP1, p27, Rpn4, MGC8644, Kip1, Cdki1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, CDKN1B

Epitope: C-Term

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN184587

Produktbeschreibung

Weitere Bezeichnung: P27KIP1 / CDKN1B (C Terminus)

Gen-ID: 1027, 12576 (mouse), 83571 (rat)

Immunogen: C-EQTPKKPGLRRRQT

Format: Affinity-purified

Beschreibung: All names and symbols: CDKN1B, P27KIP1, cyclin-dependent kinase inhibitor 1B (p27, Kip1), KIP1, CDKN4, cyclin-dependent kinase inhibitor 1B, p27(KIP1), KIP1, p27. Official Symbol: CDKN1B. RefSeq number(s): NP_004055.1. Gene ontology terms: cyclin-dependent protein kinase inhibitor activity, protein binding, transforming growth factor beta receptor, cytoplasmic mediator activity, cell cycle, cell cycle arrest, negative regulation of cell proliferation, regulation of cyclin-dependent protein kinase activity,

Spezifität: Human, Mouse, Rat, Cow, Dog

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:32,000. Approx 26kDa band observed in Human Breast and Prostate lysates (calculated MW of 22.1kDa according to NP_004055.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xia et al, Proc Natl Acad Sci U S A. 2005 Sep 27,102(39):14028-33, PMID: 16169901). Recommended concentration: 1-3µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN184587 (1µg/ml) staining of Human Breast lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Toyoshima, Hunter: "p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21." in: Cell, Vol. 78, Issue 1, pp. 67-74, 1994 (PubMed).