Collagen, Type IV (COL4) Antikörper

Antigen: Collagen, Type IV (COL4)

Synonyme: 1209, 6072, CT25584, DmColA2, alpha(IV)2/vkg, coll. IV, l(2)01209, DmelCG16858, CG16858, CG25C, CT12803, Cgc25, Cgc25C, DCg1, Dcg, alpha(IV)1/Cg25C, alpha1, gp125, DmelCG4145, CG4145

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human, Rind (Kuh)

Applikation: Immunhistochemie (IHC), ELISA

Produktnummer: ABIN135052

Produktbeschreibung

Weitere Bezeichnung: Type IV Collagen

Format: Purified

Beschreibung: Reacts with conformational determinants on human type IV collagen as demonstrated by ELISA. May react with type IV collagen from other species. Exhibits <10% cross reactivity with collagen type I, II, III, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

Anwendungen

Applikationshinweise: WORKING DILUTIONS Immunocytochemistry/ Immunohistochemistry: Purified (1:10-1:20), Biotin (1:20-1:40). ELISA: Biotin (1:1000-1:4000). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need. *Please note that harsh chemicals or procedures involving heat may denature collagen epitopes needed for proper binding. We recommend avoiding such applications when using this antibody.

Lagerung: The purified (UNLB) antibody is supplied as 0.2 mg of purified immunoglobulin in 0.5 mL (C = 0.4 mug/mL) of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.2 mg in 0.5 mL (C = 0.4 mug/mL) of PBS/NaN3. Store at 2-8C. Reagents are stable for the period shown on the label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Thallas-Bonke, Coughlan, Bach et al.: "Preservation of Kidney Function with Combined Inhibition of NADPH Oxidase and Angiotensin-Converting Enzyme in Diabetic Nephropathy." in: American journal of nephrology, Vol. 32, Issue 1, pp. 73-82, 2010 (PubMed).

Manigrasso, Sawyer, Marbury et al.: "Inhibition of estradiol synthesis attenuates renal injury in male streptozotocin-induced diabetic rats." in: American journal of physiology. Renal physiology, Vol. 301, Issue 3, pp. F634-40, 2011 (PubMed).

Boyette-Davis, Cata, Zhang et al.: "Follow-up psychophysical studies in bortezomib-related chemoneuropathy patients." in: The journal of pain : official journal of the American Pain Society, Vol. 12, Issue 9, pp. 1017-24, 2011 (PubMed).

Liu, Chen, Huang et al.: "C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes." in: Diabetologia, Vol. 54, Issue 10, pp. 2713-23, 2011 (PubMed).

Glorieux, Bachert, Favoreel et al.: "Herpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa." in: PLoS ONE, Vol. 6, Issue 7, pp. e22160, 2011 (PubMed). Von den Autoren verwendete Methode: Immunohistochemistry (IHC) (1:50). Weitere Details: Cryosections were incubated (1 h, 37

Manigrasso, Sawyer, Hutchens et al.: "Combined inhibition of aromatase activity and dihydrotestosterone supplementation attenuates renal injury in male streptozotocin (STZ)-induced diabetic rats." in: American journal of physiology. Renal physiology, 2012 (PubMed).