Checkpoint Kinase 1 (CHEK1) Antikörper

Antigen: Checkpoint Kinase 1 (CHEK1)

Synonyme: CHK1, Chk1, rad27, C85740, CHEK1, LOC398191, chk1, chek1

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Hefe (Saccharomyces Cerevisiae)

Applikation: Immunhistochemie (IHC), ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129667

Produktbeschreibung

Weitere Bezeichnung: CHK1

Gen-ID: NP_009833

Swiss-Prot: P38147

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with asynthetic peptide corresponding aa 312-327 of S. cerevisiae CHK1. Protein Sequence: Yeast CHK1, 527 aa, predicted MW 60.0 kDa 1 mslsqvsplp hikdvvlgdt vgqgafacvk nahlqmdpsi ilavkfihvp tckkmglsdk 61 ditkevvlqs kcskhpnvlr lidcnvskey mwiilemadg gdlfdkiepd vgvdsdvaqf 121 yfqqlvsain ylhvecgvah rdikpenill dkngnlklad fglasqfrrk dgtlrvsmdq 181 rgsppymape vlyseegyya drtdiwsigi llfvlltgqt pwelpslene dfvffiendg 241 nlnwgpwski efthlnllrk ilqpdpnkrv tlkalklhpw vlrrasfsgd dglcndpell 301 akklfshlkv slsnenylkf tqdtnsnnry istqpignel aelehdsmhf qtvsntqraf 361 tsydsntnyn sgtgmtqeak wtqfisydia alqfhsdend cnelvkrhlq fnpnkltkfy 421 tlqpmdvllp ilekalnlsq irvkpdlfan ferlcellgy dnvfpliini ktksnggyql 481 cgsisiikie eelksvgfer ktgdplewrr lfkkisticr diilipn

Format: Liquid (sterile filtered)

Beschreibung: CHK1 (also known as serine/threonine-protein kinase CHK1 and checkpoint kinase 1) isinvolved in cell cycle arrest when DNA damage has occurred or when unligated DNA is present. The kinase controlsphosphorylation and abundance of PDS1 to prevent anaphase entry. Also helps prevent mitotic exit. CHK1 is localizedwithin the nucleus.

Spezifität: This affinity purified antibody is directed against yeast CHK1 protein. The product was affinitypurified from monospecific antiserum by immunoaffinity purification. Reactivity occurs against yeast CHK1 protein. ABLAST analysis was used to suggest little to no cross reactivity with this protein from other sources.

Anwendungen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA and by western blot. Specificconditions for reactivity should be optimized by the end user. Expect a predominant band approximately 40-60 kDa insize corresponding to CHK1 by western blotting in the appropriate cell lysate or extract. ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:200 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized.

Konzentration: 0.73 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storagealiquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) yearfrom date of opening.

Forschungsgebiet: Kinasen/Phosphatasen, Zellzyklus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Checkpoint Kinase 1 (CHEK1) antibody