Proliferating Cell Nuclear Antigen (PCNA) Antikörper

Antigen: Proliferating Cell Nuclear Antigen (PCNA)

Synonyme: MGC8367, PCNAR, Pcna/cyclin, pcna

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129658

Produktbeschreibung

Gen-ID: AAF67834

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizationswith a synthetic peptide corresponding to amino acids 55-71 of Isochrysis galbana PCNA.

Format: Liquid (sterile filtered)

Beschreibung: PCNA, or Proliferating CellNuclear Antigen, is an auxiliary protein of DNApolymerase delta and is involved in the controlof eukaryotic DNA replication by increasing thepolymerase's processibility during elongationof the leading strand. PCNA is a nuclearprotein.

Spezifität: This affinity purified antibody is directed against algal PCNA. The product was affinity purifiedfrom monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibodywould react with PCNA from the following alga sources: Prorocentrum minimum, Tetraselmis chui, Pleurochrysiscarterae and Isochrysis galbana. Expect partial reactivity with PCNA from other alga sources as well as carrot, tobacco,peas, soybean and rice based on partial protein sequence homology. Species that have been tested but yielded nopositive results are Pfiesteria piscicida, Emiliania huxleyi, Akashiwo sanguinea, Rhodomonas sp., Karlodinium micrum,and Skletonema costatum. Reactivity with PCNA from other sources has not been determined.

Anwendungen

Applikationshinweise: Recommended Dilutions: ELISA 1:10,000 - 1:50,000 WESTERN BLOT 1:200 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized. Note: This affinity purifiedantibody has been tested for use in ELISA andwestern blot. Specific conditions for reactivityshould be optimized by the end user. Expect aband at ~29 kDa in size corresponding toPCNA by western blotting in the appropriatecell lysate or extract.

Konzentration: 1.33 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storagealiquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) yearfrom date of opening.

Forschungsgebiet: Proliferationsmarker

Beschränkungen: Nur für Forschungszwecke einsetzbar