Polo-Like Kinase 1 (PLK1) (pT210) Antikörper

Antigen: Polo-Like Kinase 1 (PLK1)

Synonyme: PLK, STPK13, Snk, plk, MGC52566, Plx1, PLK-1, stpk13, MGC69251, MGC133927

Epitope: pT210

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunhistochemie (IHC), ELISA, Western Blot (WB)

Produktnummer: ABIN129581

Produktbeschreibung

Weitere Bezeichnung: Polo-like Kinase (Plk1) pT210

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho peptide corresponding aa 205-214 of Human Polo-like kinase 1 (Plk1) protein.

Format: Liquid (sterile filtered)

Beschreibung: Anti-Polo-like Kinase (Plk1) pT210 (RABBIT) Antibody. Background: Polo-like kinase 1, also known as Plk-1, serine-threonine protein kinase 13 and STPK13, may be required for cell division and have a role during G1 or S phase and is associated with the phosphorylation of cyclin B1. Phosphorylation of Plk-1 on T210 contributes to proper mitotic progression. Plk-1 has a nuclear localization and accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. Plk-1 is a member of the Ser/Thr protein kinase family and the CDC5/Polo subfamily and contains 2 POLO box domains.

Spezifität: This antibody is specific for phosphorylated human Plk-1 protein at the pT210 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Plk-1 homologues from human, chimpanzee, pig, chicken, mouse, rat, Xenopus, dog, mosquito, zebra fish, starfish, sea urchin and fruit fly. Cross reactivity with Plk-1 protein homologues from C.elgans and honeybee may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with Plk-1 protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.

Anwendungen

Applikationshinweise: This affinity-purified antibody has been tested for use in ELISA, IHC and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 68 kDa in size corresponding to Plk-1 by western blotting in the appropriate cell lysate or extract. This antibody is positive by IHC on kidney, liver, cancer, thyroid and lymphocyte tissue. ELISA: 1:3,000 - 1:12,000. WB: 1:200 - 1:2,000. IHC: 1:200 - 1:1,000.

Konzentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Reinheit: This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Lagerung: Store vial at -20° C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.

Forschungsgebiet: Kinasen/Phosphatasen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Adler, Mueller, Rached et al.: "Modulation of key regulators of mitosis linked to chromosomal instability is an early event in ochratoxin A carcinogenicity." in: Carcinogenesis, Vol. 30, Issue 4, pp. 711-9, 2009 (PubMed).