CD25 Antikörper

Produktnummer ABIN120428

Der Maus Monoklonal anti-CD25 Antikörper wird verwendet zum Nachweis von CD25 in Proben von Ratte. Er wurde validiert für FACS, IP, IHC (fro), IHC (p) und EIA.

Kurzübersicht für CD25 Antikörper (ABIN120428)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD25 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Klon: OX-39

Produktdetails anti-CD25 Antikörper

Spezifität: This antibody recognizes the alpha chain of CD25, otherwise known as IL-2 receptor alpha. This clone has been described reacting with paraffin-embedded material following PLP fixation (periodatelysine-paraformaldehyde). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts. We recommend the use of SM265LE for this purpose.

Aufreinigung: Affinity chromatography on Protein G

Immunogen: Stimulated Rat T cells. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/1 mouse myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. ELISA. Immunoprecipitation. Immunohistochemistry on frozen and paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.02 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Andere Bezeichnung: CD25 / IL2RA

Hintergrund: CD25 is a 55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55

Gen-ID: 25704

NCBI Accession: NP_037295

UniProt: P26897

Pathways: JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation