c-MYC Antikörper (C-Term)

Produktnummer ABIN119684

Der Maus Monoklonal anti-c-MYC Antikörper wird verwendet zum Nachweis von c-MYC in Proben von Human. Er wurde validiert für WB, FACS, IP, IHC (p), IHC (fro) und EIA.

Kurzübersicht für c-MYC Antikörper (C-Term) (ABIN119684)

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser c-MYC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: 9-00E-010

Produktdetails anti-c-MYC Antikörper

Bindungsspezifität: C-Term

Sequenz: EQKLISEEDL

Spezifität: This antibody detects the 62 kDa c-myc gene product, which is involved in the regulation of the cell cycle and cell growth. This antibody may also be used to detect the commonly used c-myc tag.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: Synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of Human c-myc. Spleen cells from immunised Balb/c mice were fused with cells of the myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: 1/100-1/500. Western Blot: 1/100-1/500 (Non-Reducing Conditions). Detects a band of approximately 62 kDa in Colo 320HSR cell lysates. Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 1x10^6 cells in 100 μL. Membrane permeabilisation is required. Immunohistochemistry on Frozen and Paraffin Sections: This product does not requireprotein digestion pre-treatment of paraffin sections prior to staining. It does not requireantigen retrieval using heat treatment prior to staining of paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Andere Bezeichnung: C-Myc

Hintergrund: P62c-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.Synonyms: c-myc tag, myc tag, myc-tag

Gen-ID: 4609

NCBI Accession: NP_002458

UniProt: P01106

Pathways: p53 Signalweg, Zellzyklus, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effekt