Granzyme B (Granzyme 2, Cytotoxic T-Lymphocyte-Associated serine Esterase 1) (Gzmb) Antikörper

Antigen: Granzyme B (Granzyme 2, Cytotoxic T-Lymphocyte-Associated serine Esterase 1) (Gzmb)

Synonyme: HLP, CCPI, CGL1, CSPB, SECT, CGL-1, CSP-B, CTLA1, CTSGL1, GZB, CCP1, Ctla1, Ctla-1, AI553453, CCP-1/C11, RNKP-1, MGC156565, GZMB

Klonalität: Monoklonal (GM4C1)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Zell-ELISA (cELISA), ELISA

Produktnummer: ABIN108719

Produktbeschreibung

Weitere Bezeichnung: Granzyme B

Immunogen: GM4C1 was generated by genetic immunisation of BALB/c mice with an immunisation vector containing the granzyme B cDNA. It was purified by affinity chromatography from cell culture supernatants and is routinely tested by flow cytometry on BOSC cells trans

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: GM4C1

Beschreibung: Granzymes are exogenous serine proteases that are stored in the cytotoxic granules of activated T cells and NK cells. Upon target cell contact, the contents of these granules are directionally exocytosed and, with the assistance of perforin, the granzymes enter the cytosol of the target cell. GM4C1 reacts with human granzyme B (GrB), a 27 kDa serine protease which cleaves target-cell proteins at specific aspartate residues. In the target cell, granzyme B rapidly induces apoptosis by activating both caspase-mediated as well as caspase-independent pathways. Apart from the storage granules of cytotoxic cells granzymes can also be detected in biological fluids such as serum and plasma. Elevated levels of soluble granzymes have been found in the sera of patients with increased cytotoxic responses, caused by various viral infections, as well as in the synovial fluid of rheumatic arthritis patients. A high percentage of GrB positive CTL has also been found in the glands of patients suffering from Hodgkin`s disease and in CTL infiltrates in transplant rejection.

Spezifität: GM4C1 recognises granzyme B transiently expressed on the cell surface of transfected BOSC cells (Fig. 1) as well as the native protein in peripheral blood mononuclear cells (Fig. 2). The antibody does not cross-react with the human granzymes A, K or M (Fi

Anwendungen

Applikationshinweise: GM4C1 has been tested for the use in flow cytometry, ELISA and cell-ELISA. It is expected that the antibody will be suited for all applications where recognition of the native protein is required. A titration should be performed to determine the optimal

Lagerung: Store the antibody at 4°C. Avoid repeated freezing and thawing.

Forschungsgebiet: Apoptose/Nekrose, Apoptose/Nekrose, Inflammation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Specificity testing of GM4C1. BOSC cells were transiently transfected with expression vectors for granzyme A, B, K and M as well as an irrelevant protein. Expression of the constructs was tested with an anti-tag monoclonal antibody (B, open curves)

Intracellular detection of granzyme B in human PBMC Mononuclear cells from peripheral blood (PBMC) were seperated by Ficoll-Hypaque, cultivated for 6h in the presence of phorbolester and ionomycin, fixed and permeabilised. Cells were incubated with hybr