TNFRSF1A Antikörper

Produktnummer ABIN105278

Hersteller Produkt- Nr.: 209-401-308

Der Kaninchen Polyklonal anti-TNFRSF1A Antikörper wird verwendet zum Nachweis von TNFRSF1A in Proben von Human. Er wurde validiert für WB, IP, ELISA und FACS.

Kurzübersicht für TNFRSF1A Antikörper (ABIN105278)

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TNFRSF1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Flow Cytometry (FACS)

Produktdetails anti-TNFRSF1A Antikörper

Hersteller Produkt- Nr.: 209-401-308

Hersteller: Rockland

Verwendungszweck: TNF p55 Receptor Antibody

Kreuzreaktivität (Details): This antibody is directed against the extracellular domain of the cell bound human TNFRp55.

Produktmerkmale: Synonyms: rabbit anti-TNFp55 Receptor Antibody, TBP1 antibody, TNFR1 antibody, TNFR55 antibody, TNFRSF1A antibody, Tumor necrosis factor alpha receptor antibody, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, TNF-R1, Tumor necrosis factor receptor type I, TNF-RI, TNFR-I, p55, p60, CD120a, Tumor necrosis factor receptor superfamily member 1A membrane form, Tumor necrosis factor-binding protein 1, TBPI , TNFRSF1A, TNFAR, TNFR1

Aufreinigung: Anti-Human TNF p55 Receptor Antibody is an IgG preparation of whole rabbit serum purified by Protein G chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human sTNFRp55 expressed in Chinese Hamster Cells.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Flow Cytometry Dilution: User Optimized

Application Note: Anti-TNF p55 Receptor (sTNFRp55) antibody has been tested for use in ELISA,  radioimmunoassays, immunoblotting, flow cytometry and immunoprecipitation.  Reactivity in other immunoassays is unknown. This product has been assayed for the ability to immunoprecipitate antigen and a dilution range of 1:400 to 1:800 is suggested.  For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background.  This product has been assayed by immunoblot and a working dilution range of 1:200 to 1:400 is suggested for this application.  This product has been assayed by ELISA against soluble (extracellular) sTNFRp55 at a dilution range of 1:2,000 to 1:8,000.  For use in ELISA formats, this antibody is best used as the second antibody  in combination with a monoclonal antibody as a capture antibody.  This product has been assayed by radioimmunoassay against antigen.  A dilution of 1:8,000 is suggested for this immunoassay.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu TNFRSF1A

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Andere Bezeichnung: TNFRSF1A

Hintergrund: Background: Human TNF p55 Receptor Antibody recognizes TNFR1, the p55 soluble TNFR protein. sTNFRp55 is one of the two transmembrane receptors for tumor necrosis factor (TNF) with different molecular weight, 55 kD (p55) and 75 kD (p75), have been identified. The extracellular part of both receptors are shedded by proteolytic cleavage and exist as soluble receptors which bind to TNF in plasma and other biological fluids. sTNFR proteins have been implicated in certain leukemias. Anti-sTNFRp55 antibody is suitable for researchers in Cancer, Immunology, and Cell Biology.

Gen-ID: 7132

UniProt: P19438

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Hepatitis C, Ubiquitin Proteasome Pathway