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Leptin (LEP) Antikörper

Antigen

Leptin (LEP)
Synonyme OB, OBS, FLJ94114, ob, obese
Klonalität Polyklonal
Wirt
Alternativen

Kaninchen
Reaktivität
Applikation
Alternativen Immunhistochemie (IHC), ELISA, Western Blot (WB), Immunpräzipitation (IP)
Produktnummer ABIN103522
Menge 1mg  (1.0 mg/ml (by UV absorbance at 280 ...)  (Varianten)
Preis 315,11 €   Zzgl. Versandkosten €40,00 und MWSt
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Verfügbarkeit Lieferung in 5 Werktagen

Produktbeschreibung
Anwendungen Alternativen Sekundärantikörper Varianten zurück nach oben

Weitere Bezeichnung Leptin
Immunogen The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant mouse leptin 16,000 MW produced in E. coli.
Format Liquid (sterile filtered)
Spezifität This is an IgG preparation of whole rabbit serum purified by Protein G chromatography. The antibody will recognize recombinant and native 16 kDa leptin from mouse. Reactivity with leptin from other sources is unknown. Endotoxin Content: <10 pg/ ul by LAL method.

Anwendungen
Alternativen Sekundärantikörper Varianten zurück nach oben

Applikationshinweise Recommended Dilutions: This product has been assayed by immunoblot against tissue homogenates using HRP Goat-anti-Rabbit IgG [H&L] (code ABIN101990) and TMB as a substrate. A working dilution range of 1:200 to 1:400 is suggested for this application. This product has been assayed by ELISA against recombinant mouse leptin using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code ABIN101990) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:2,400 is suggested for this product. Optimal titers for other applications should be determined by the researcher. Note: This IgG fraction of anti-Mouse Leptin has been tested for use in ELISA and immunoblotting. Reactivity in other immunoassays is unknown. Obesity associated leptin is the product of the OB gene and has been identified with Type II diabetes.
Konzentration 1.0 mg/ml (by UV absorbance at 280 nm)
Buffer Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.
Lagerung Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is six (6) months from date of opening.
Forschungsgebiet Kardiovaskuläres System, Artherosklerose, Hormone
Beschränkungen Nur für Forschungszwecke einsetzbar