IL1R2 Antikörper

Produktnummer ABIN103286

Hersteller Produkt- Nr.: 209-401-305

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch IL1R2 in WB, ELISA, FACS und IP. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für IL1R2 Antikörper (ABIN103286)

Target: IL1R2 (Interleukin 1 Receptor, Type II (IL1R2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL1R2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP)

Produktdetails anti-IL1R2 Antikörper

Hersteller Produkt- Nr.: 209-401-305

Hersteller: Rockland

Verwendungszweck: IL-1 Receptor Type II Antibody

Kreuzreaktivität (Details): The antibody is directed against the extracellular domain of the human IL-1R type II, but precipitates the intact receptor expressed on human cells.

Produktmerkmale: Synonyms: rabbit anti-IL-1 Receptor Type II Antibody, rabbit anti-interleukin-1 receptor beta type II antibody, IL1R Beta antibody, Il1r-2 antibody, IL1R2 antibody, IL1RB antibody, Interleukin 1 receptor beta type II antibody

Aufreinigung: This is an IgG preparation of whole rabbit serum purified by Protein A chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1R type II.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Flow Cytometry Dilution: User Optimized

Application Note: This IgG fraction antibody of anti-Human IL-1 Receptor Type II (IL-1RII) has been tested for use in ELISA and Western Blot and is suitable for use in radioimmunoassays, flow cytometry and immunoprecipitation. Reactivity in other immunoassays is unknown.  IL-1RII has been termed p68 or CD121 in some laboratories.  IL-1RII has been shown to bind IL-1, IL-1a and IL-1b. This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (code # 611-1302) and TMB as a substrate.  A working dilution range of 1:200 to 1:400 is suggested for immunoblot. This product has been assayed by ELISA against soluble (extracellular) IL-1RII using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature.  A working dilution range of 1:1,000 to 1:2000 is suggested for ELISA.  For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. A dilution range of 1:400 to 1:800 is suggested for immunoprecipitation.  For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background.  A dilution of 1:8,000 is suggested for radioimmunoassay. 

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu IL1R2

Target: IL1R2 (Interleukin 1 Receptor, Type II (IL1R2))

Andere Bezeichnung: IL1R2

Hintergrund: Background: Interleukin 1 receptor type II binds to IL-1 alpha, IL-1 beta and IL1R1/IL1RA. Anti-IL-1 Receptor Type II antibody is ideal for investigators involved in Immunology and Stem Cell research.

Gen-ID: 7850

UniProt: P27930

Pathways: NF-kappaB Signalweg