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- Wirt
- Maus
- Applikation
- Western Blotting (WB)
- Produktmerkmale
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The mouse tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different mouse tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane.The mouse tissues included in the blot are adrenal, cerebellum, cerebrum, small intestine, skeletal muscle, stomach, testis, and thymus.
Sample diagnosis: Normal
Samples loaded: Lanes: 1: protein markers, 2: adrenal, 3: cerebellum, 4: cerebrum, 5: small intestine, 6: skeletal muscle, 7: stomach, 8: testis, and 9: thymus.
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
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Rat Tail
Wirt: Ratte
Sheep Red Blood Cells (10% Washed Pooled Cells)H Wirt: Schaf Normal Red Blood Cells
Mouse BrainIEM, IHC, WB Wirt: Maus Normal Brain
Hamster Complement (Lyophilized) With DiluentWirt: Hamster Normal Complement
Chicken Red Blood Cells (10% Washed Pooled Cells)H Wirt: Huhn Normal Red Blood Cells
Guinea Pig Complement (Lyophilized) With DiluentWirt: Meerschweinchen Normal Complement
Rabbit Blood (In Sodium Citrate)H Wirt: Kaninchen Normal Blood
Sheep Red Blood Cells (100% Washed Pooled Cells)H Wirt: Schaf Normal Red Blood Cells
Guinea Pig Complement (Fresh Frozen)Wirt: Meerschweinchen Normal Complement
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- Applikationshinweise
- For Western Blot, the blot can be blocked with 1 % BSA or 5 % nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The blot can be reused after stripping.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store at 4 °C.
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