Ziege anti-Maus IgG (Fc Region) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed

Produktnummer ABIN965174

Hersteller Produkt- Nr.: 710-1522

Kurzübersicht für Ziege anti-Maus IgG (Fc Region) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed (ABIN965174)

Target: IgG

Fragment: F(ab')2 fragment

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Fc Region

Reaktivität: Maus

Wirt: Ziege

Hersteller Produkt- Nr.: 710-1522

Hersteller: Rockland

Verwendungszweck: F(ab')2 Mouse IgG Fc Antibody Alkaline Phosphatase Conjugated Antibody Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv Hs & Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab) or Bovine, Horse and Human Serum Proteins.

Produktmerkmale: F(ab')2 Anti-Mouse IgM (mu chain) Antibody generated in Goat detects specifically Mouse IgM mu heavy chain. F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Sterilität: Sterile filtered

Immunogen: Optional[Immunogen]: Mouse IgG F(c) fragment

Anwendungsinformationen

Applikationshinweise: Application Note: Fab')2 Anti-Mouse IgG F(c) Alkaline Phosphatase Antibody has been tested by ELISA and is assayed against 1.0 μg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: Optional[Buffer]: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: F(ab')2 Anti-Mouse IgG F(c) Alkaline Phosphatase Antibody was generated in goat and detects specifically Mouse IgG F(c). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.