Maus anti-Human IgE Antikörper

Produktnummer ABIN94398

Kurzübersicht für Maus anti-Human IgE Antikörper (ABIN94398)

Target: IgE

Klonalität: Monoklonal

Applikation: ELISA, Flow Cytometry (FACS)

Klon: 4G7-325

Isotyp: IgG

Produktdetails

Reaktivität: Human

Wirt: Maus

Verwendungszweck: Anti-Hu IgE Purified

Kreuzreaktivität (Details): Human

Produktmerkmale: The mouse monoclonal antibody 4G7.325 reacts with human IgE, it recognizes an epitope different from the ones recognized by BE5 and 4H10 antibodies to IgE. The epitope is located within the amino acids 103-115 (WSDYNFDYSSSEE).

Aufreinigung: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Purified human IgE.

Anwendungsinformationen

Applikationshinweise: Flow cytrometry: Recommended dilution: 1-4 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen

Smith, Benjamin, Hozic, Derewenda, Smith, Thomas, Gafvelin, van Hage-Hamsten, Chapman: "The molecular basis of antigenic cross-reactivity between the group 2 mite allergens." in: The Journal of allergy and clinical immunology, Vol. 107, Issue 6, pp. 977-84, (2001) (PubMed).

Mueller, Smith, Chapman, Rule, Benjamin: "Hydrogen exchange nuclear magnetic resonance spectroscopy mapping of antibody epitopes on the house dust mite allergen Der p 2." in: The Journal of biological chemistry, Vol. 276, Issue 12, pp. 9359-65, (2001) (PubMed).

Antigendetails

Target: IgE

Hintergrund: Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E

Molekulargewicht: 180 kDa

Gen-ID: 35