Maus anti-Human IgE Antikörper

Produktnummer ABIN94399

Kurzübersicht für Maus anti-Human IgE Antikörper (ABIN94399)

Target: IgE

Klonalität: Monoklonal

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Klon: 4H10

Isotyp: IgG1

Produktdetails

Reaktivität: Human

Wirt: Maus

Verwendungszweck: Anti-Hu IgE Purified

Kreuzreaktivität (Details): Human

Produktmerkmale: The mouse monoclonal antibody 4H10 reacts with human IgE, it recognizes an epitope different from the ones recognized by BE5 and 4G7.325 antibodies to IgE. The epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL).

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Purified human IgE.

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 4 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Antigendetails

Target: IgE

Substanzklasse: Antibody

Hintergrund: Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E

Molekulargewicht: 180 kDa

Gen-ID: 35