Ziege anti-Maus IgG (Heavy & Light Chain) Antikörper (Poly-HRP)

Produktnummer ABIN7849474

Kurzübersicht für Ziege anti-Maus IgG (Heavy & Light Chain) Antikörper (Poly-HRP) (ABIN7849474)

Target: IgG

Klonalität: Polyklonal

Konjugat: Poly-HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunochromatography (IC)

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Maus, Kaninchen

Wirt: Ziege

Verwendungszweck: Goat Polyclonal Secondary Antibody to Mouse/Rabbit IgG (H&L) HRP polymer labeled

Spezifität: By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and Rabbit IgG. No antibody was detected against non immunoglobulin serum proteins.

Aufreinigung: Goat Polyclonal Secondary Antibody to Mouse/Rabbit IgG (H&L) have been cross-adsorbed against IgG from bovine, goat, horse and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

Immunogen: Mouse/Rabbit IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/5000 - 1/20000, WB: 1/5000 - 1/20000, IH: 1/100 - 1/500, IC: 1/100 - 1/500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 5 mg/mL

Buffer: Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1 % BSA, 50 % glycerol, 0.05 % Proclin

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Antigendetails

Target: IgG

Substanzklasse: Antibody