Maus anti-Human IgA2 (Allotype A2m2) Antikörper

Produktnummer ABIN7674778

Kurzübersicht für Maus anti-Human IgA2 (Allotype A2m2) Antikörper (ABIN7674778)

Target: IgA2

Klonalität: Monoklonal

Applikation: ELISA, Western Blotting (WB), Indirect Immunofluorescence Assay (IFA)

Klon: NI 194-3 (A89-040)

Isotyp: IgG1

Produktdetails

Bindungsspezifität: Allotype A2m2

Reaktivität: Human

Wirt: Maus

Verwendungszweck: Monoclonal Mouse antiSerum to Human IgA2(m)2, allotype specific

Spezifität: Monoclonal Mouse antiSerum to Human IgA2(m)2, allotype specific

Kreuzreaktivität (Details): The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.

Produktmerkmale: The reactivity of the antiserum is restricted to the IgA2(m)2 Molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgA2(m)2, haemagglutination and immunoblotting.

Aufreinigung: Delipidated, heat inactivated whole ascites

Immunogen: Highly purified monoclonal IgA2(m)2 isolated from human serum.

Anwendungsinformationen

Applikationshinweise: Indirect immunofluorescence,ELISA. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:200 upwards, in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1 mg/mL. No foreign proteins added. Reconstitute the lyophilized ascites by adding 0.5 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1 mg/mL. No foreign proteins added.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day.

Antigendetails

Target: IgA2