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Maus anti-Human IgA2 (IgA2(m)2) Antikörper (Biotin)

ELISA, WB, ICC, IHC (p), DB Monoclonal NI 194-3 (A89-040) IgG1 Biotin
Produktnummer ABIN457865
  • Target Alle IgA2 Produkte
    IgA2
    Bindungsspezifität
    • 4
    • 4
    • 1
    IgA2(m)2
    Reaktivität
    Human
    Wirt
    • 11
    • 2
    Maus
    Klonalität
    • 11
    • 2
    Monoklonal
    Konjugat
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Biotin
    Applikation
    • 10
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)
    Spezifität
    Biotin-conjugated purified monoclonal Mouse antibody to Human IgA2(m)2, allotype specific
    Kreuzreaktivität (Details)
    The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.
    Produktmerkmale
    The reactivity of the antiserum is restricted to an allotype specific determinant on IgA2(m)2 Molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA2(m)2, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. The optimum working dilution is an assayrelated characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:200 upwards, in Western blotting from 1:200 upwards.
    Aufreinigung
    Purified
    Immunogen
    Highly purified monoclonal IgA2(m)2 isolated from human serum.
    Klon
    NI 194-3 (A89-040)
    Isotyp
    IgG1
  • Applikationshinweise
    ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.
    Buffer
    Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
    Konservierungsmittel
    Without preservative
    Lagerung
    RT,4 °C,-20 °C
    Informationen zur Lagerung
    The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.
  • Target
    IgA2
    Andere Bezeichnung
    IgA22 (IgA2 Produkte)
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