Kaninchen anti-Maus IgG (Fc Region) Antikörper (DyLight 800) - Preadsorbed

Produktnummer ABIN6699051

Hersteller Produkt- Nr.: 610-445-003

Kurzübersicht für Kaninchen anti-Maus IgG (Fc Region) Antikörper (DyLight 800) - Preadsorbed (ABIN6699051)

Target: IgG

Klonalität: Polyklonal

Konjugat: DyLight 800

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Fc Region

Reaktivität: Maus

Wirt: Kaninchen

Hersteller Produkt- Nr.: 610-445-003

Hersteller: Rockland

Produktmerkmale:

Synonyms: Rabbit Anti-Mouse IgG F(c) Antibody DyLight™ 800 Conjugation, Rabbit Anti-Mouse IgG Fc Antibody DyLight™ 800 Conjugated, Rabbit Anti-Mouse IgG Fc Fragment DyLight™800 Conjugated Antibody

Background: Anti-Mouse IgG F(c) DyLight antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.

Aufreinigung: Preadsorption: immunoaffinity chromatography using Mouse IgG coupled to agarose beads

Immunogen:

Immunogen: Anti-Mouse IgG F(c) was produced by repeated immunization with mouse IgG F(c) fragment in rabbit.

Immunogen Type: Native Protein

Labeling Ratio: 1.9

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Mouse IgG F(c) DyLight™800 Antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

ELISA Dilution: 1:40,000 - 1:180,000

Western Blot Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: >1:5,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody