Ziege anti-Maus IgG Antikörper (DyLight 800)

Produktnummer ABIN6699035

Hersteller Produkt- Nr.: 610-145-002

Kurzübersicht für Ziege anti-Maus IgG Antikörper (DyLight 800) (ABIN6699035)

Target: IgG

Klonalität: Polyklonal

Konjugat: DyLight 800

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotyp: IgG

Produktdetails

Reaktivität: Maus

Wirt: Ziege

Hersteller Produkt- Nr.: 610-145-002

Hersteller: Rockland

Verwendungszweck: Mouse IgG (H&L) Antibody DyLight™ 800 Conjugated

Produktmerkmale: Goat Anti-Mouse IgG Secondary Antibody DyLight™800 Conjugated, Goat Anti-Mouse IgG Antibody DyLight™800 Conjugated, Anti-mouse IgG secondary antibody, anti-mouse IgG DyLight™800 conjugated secondary antibody,Anti-Mouse IgG DyLight 800 Antibody generated in goat detects reactivity to Mouse IgG.

Aufreinigung:

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. This antibody will react with heavy chains of Mouse IgG and with light chains of most Mouse immunoglobulins.

Immunogen: Mouse IgG whole molecule

Labeling Ratio: 1.6

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Kommentare:

Anti-Mouse IgG DyLight 800 Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, IHC, IP

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Li, Krafft, Zeng, Duan, Qi, Shao, Xue, Zhang: "Microglia Autophagy Mediated by TMEM166 Promotes Ischemic Stroke Secondary to Carotid Artery Stenosis." in: Aging and disease, Vol. 15, Issue 3, pp. 1416-1431, (2024) (PubMed).

Voß, Klaus, Lichti, Ganter, Guizetti: "Malaria parasite centrins can assemble by Ca2+-inducible condensation." in: PLoS pathogens, Vol. 19, Issue 12, pp. e1011899, (2024) (PubMed).

Wu, Wang, Liang, Chen, Sun, Wei, Zhong, Huang, Liu, Wang, Yu, Lan: "SARS-CoV-2 N protein induced acute kidney injury in diabetic db/db mice is associated with a Mincle-dependent M1 macrophage activation." in: Frontiers in immunology, Vol. 14, pp. 1264447, (2023) (PubMed).

Zhang, Shi, Liu, Chen, Zhang, Zhang, Cong, Li: "Interleukin 6 (IL-6) Regulates GABAA Receptors in the Dorsomedial Hypothalamus Nucleus (DMH) through Activation of the JAK/STAT Pathway to Affect Heart Rate Variability in Stressed Rats." in: International journal of molecular sciences, Vol. 24, Issue 16, (2023) (PubMed).

Cuevas-Navarro, Wagner, Van, Swain, Mo, Columbus, Allison, Cheng, Messing, Turbyville, Simanshu, Sale, McCormick, Stephen, Castel: "RAS-dependent RAF-MAPK hyperactivation by pathogenic RIT1 is a therapeutic target in Noonan syndrome-associated cardiac hypertrophy." in: Science advances, Vol. 9, Issue 28, pp. eadf4766, (2023) (PubMed).

Lopez, Bonsor, Sale, Urisman, Mehalko, Cabanski-Dunning, Castel, Simanshu, McCormick: "The ribosomal S6 kinase 2 (RSK2)-SPRED2 complex regulates the phosphorylation of RSK substrates and MAPK signaling." in: The Journal of biological chemistry, Vol. 299, Issue 6, pp. 104789, (2023) (PubMed).

Dabrowski, Tulli, Graef: "Parallel phospholipid transfer by Vps13 and Atg2 determines autophagosome biogenesis dynamics." in: The Journal of cell biology, Vol. 222, Issue 7, (2023) (PubMed).

Arroum, Borowski, Marx, Schmelter, Scholz, Psathaki, Hippler, Enriquez, Busch: "Loss of respiratory complex I subunit NDUFB10 affects complex I assembly and supercomplex formation." in: Biological chemistry, Vol. 404, Issue 5, pp. 399-415, (2023) (PubMed).

Winter, Fresenius, Cunningham, Wei, Keys, Berg, Bott, Yadav, Ryan, Sirohi, Tripp, Barta, Agarwal, Letai, Sabatini, Wohlever, Rutter: "Collateral deletion of the mitochondrial AAA+ ATPase ATAD1 sensitizes cancer cells to proteasome dysfunction." in: eLife, Vol. 11, (2023) (PubMed).

Tao, Zhu, Diaz-Perez, Yu, Foley, Stewart, Casero, Steet, Zhai: "Phenylbutyrate modulates polyamine acetylase and ameliorates Snyder-Robinson syndrome in a Drosophila model and patient cells." in: JCI insight, Vol. 7, Issue 13, (2022) (PubMed).

Wang, Bergholz, Ding, Lin, Kabraji, Hughes, He, Xie, Jiang, Wang, Zoeller, Kim, Roberts, Konstantinopoulos, Matulonis, Dillon, Winer, Lin, Zhao: "STING agonism reprograms tumor-associated macrophages and overcomes resistance to PARP inhibition in BRCA1-deficient models of breast cancer." in: Nature communications, Vol. 13, Issue 1, pp. 3022, (2022) (PubMed).

Orengo, Nitschke, van der Heijden, Ciaburri, Orr, Zoghbi: "Reduction of mutant ATXN1 rescues premature death in a conditional SCA1 mouse model." in: JCI insight, Vol. 7, Issue 8, (2022) (PubMed).

Watt, Cejas, DeCristo, Metzger-Filho, Lam, Qiu, BrinJones, Kesten, Coulson, Font-Tello, Lim, Vadhi, Daniels, Montero, Taing, Meyer, Gilan, Bell, Korthauer, Giambartolomei, Pasaniuc, Seo, Freedman, Ma, Ellis, Krop, Winer, Letai, Brown, Dawson, Long, Zhao, : "CDK4/6 inhibition reprograms the breast cancer enhancer landscape by stimulating AP-1 transcriptional activity." in: Nature cancer, Vol. 2, Issue 1, pp. 34-48, (2022) (PubMed).

Cuevas-Navarro, Van, Cheng, Urisman, Castel, McCormick: "The RAS GTPase RIT1 compromises mitotic fidelity through spindle assembly checkpoint suppression." in: Current biology : CB, Vol. 31, Issue 17, pp. 3915-3924.e9, (2022) (PubMed).

Doerfler, Park, Assini, Youssef, Saxena, Yaseen, De Giorgi, Chuecos, Hurley, Li, Marcovina, Bao, Boffa, Koschinsky, Lagor: "LPA disruption with AAV-CRISPR potently lowers plasma apo(a) in transgenic mouse model: A proof-of-concept study." in: Molecular therapy. Methods & clinical development, Vol. 27, pp. 337-351, (2022) (PubMed).

Zhang, Luk, Valadares, Aronis, Foukas: "Dominant Role of PI3K p110α over p110β in Insulin and β-Adrenergic Receptor Signalling." in: International journal of molecular sciences, Vol. 22, Issue 23, (2022) (PubMed).

Wang, Li, Yu, Yang, Nan, Guo, Zhao: "Intracerebral hemorrhage alters α2δ1 and thrombospondin expression in rats." in: Experimental and therapeutic medicine, Vol. 23, Issue 5, pp. 327, (2022) (PubMed).

Nuebel, Morgan, Fogarty, Winter, Lettlova, Berg, Chen, Kidwell, Maschek, Clowers, Argyriou, Chen, Wittig, Cox, Roh-Johnson, Braverman, Bonkowsky, Gygi, Rutter: "The biochemical basis of mitochondrial dysfunction in Zellweger Spectrum Disorder." in: EMBO reports, Vol. 22, Issue 10, pp. e51991, (2021) (PubMed).

Navarro, Tapia-Galisteo, Martín-García, Tarín, Corbacho, Gómez-López, Sánchez-Tirado, Campuzano, González-Cortés, Yáñez-Sedeño, Compte, Álvarez-Vallina, Sanz: "TGF-β-induced IGFBP-3 is a key paracrine factor from activated pericytes that promotes colorectal cancer cell migration and invasion." in: Molecular oncology, Vol. 14, Issue 10, pp. 2609-2628, (2021) (PubMed).

Raimo, Zura-Miller, Fezelinia, Spruce, Zakopoulos, Mohsen, Vockley, Ischiropoulos: "Mitochondrial morphology, bioenergetics and proteomic responses in fatty acid oxidation disorders." in: Redox biology, Vol. 41, pp. 101923, (2021) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.