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Ziege anti-Maus IgG Antikörper (DyLight 549)

WB, FLISA, FM, DB Polyclonal IgG DyLight 549
Rockland
Produktnummer ABIN6699011
Hersteller Produkt- Nr.: 610-142-002

Kurzübersicht für Ziege anti-Maus IgG Antikörper (DyLight 549) (ABIN6699011)

Target

IgG

Klonalität

  • 3453
  • 231
  • 16
  • 1
Polyklonal

Konjugat

  • 666
  • 423
  • 422
  • 400
  • 274
  • 262
  • 235
  • 119
  • 105
  • 72
  • 67
  • 64
  • 61
  • 58
  • 58
  • 50
  • 44
  • 43
  • 40
  • 39
  • 39
  • 39
  • 36
  • 36
  • 34
  • 32
  • 31
  • 30
  • 29
  • 29
  • 29
  • 29
  • 29
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 27
  • 26
  • 26
  • 25
  • 21
  • 21
  • 19
  • 17
  • 17
  • 16
DyLight 549

Applikation

  • 2120
  • 2053
  • 1900
  • 1873
  • 999
  • 919
  • 895
  • 520
  • 423
  • 234
  • 234
  • 149
  • 102
  • 98
  • 90
  • 84
  • 51
  • 33
  • 30
  • 28
  • 25
  • 20
  • 15
  • 11
  • 9
  • 8
  • 7
  • 7
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotyp

IgG
  • Reaktivität

    • 752
    • 705
    • 609
    • 474
    • 410
    • 244
    • 213
    • 176
    • 146
    • 141
    • 124
    • 110
    • 107
    • 101
    • 83
    • 62
    • 48
    • 33
    • 28
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus

    Wirt

    • 1786
    • 1583
    • 569
    • 284
    • 122
    • 112
    • 41
    • 32
    • 22
    • 21
    • 15
    • 11
    • 11
    Ziege

    Hersteller Produkt- Nr.

    610-142-002

    Hersteller

    Rockland

    Verwendungszweck

    Mouse IgG (H&L) Antibody Dylight™ 549 Conjugated

    Produktmerkmale

    Goat Anti-Mouse IgG Secondary Antibody DyLight™549 Conjugated, Goat Anti-Mouse IgG Antibody DyLight™549 Conjugated, Anti-mouse IgG secondary antibody, anti-mouse IgG DyLight™549 conjugated secondary antibody,Anti-Mouse IgG DyLight 549 Antibody generated in goat detects reactivity to Mouse IgG.

    Aufreinigung

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. This antibody will react with heavy chains of Mouse IgG and with light chains of most Mouse immunoglobulins.

    Immunogen

    Mouse IgG, whole molecule

    Labeling Ratio

    3.7
  • Applikationshinweise

    FLISA_Dilution: >1:20,000

    IF_Microscopy_Dilution: >1:5,000

    Western_Blot_Dilution: >1:10,000

    Other: User Optimized

    Kommentare

    Anti-Mouse IgG DyLight 549 Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
    Suggested Applications: IF, Multiplex

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration

    1.0 mg/mL

    Buffer

    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Wang, Chen, Hsiao, Tsai, Jiang, Lee, Tsai, Chen: "Insulin-like growth factor binding protein 3 promotes radiosensitivity of oral squamous cell carcinoma cells via positive feedback on NF-κB/IL-6/ROS signaling." in: Journal of experimental & clinical cancer research : CR, Vol. 40, Issue 1, pp. 95, (2021) (PubMed).

    Iguchi, Carrasco, Xie, Pineda, Malykhina, Wilcox: "Functional constipation induces bladder overactivity associated with upregulations of Htr2 and Trpv2 pathways." in: Scientific reports, Vol. 11, Issue 1, pp. 1149, (2021) (PubMed).

    Lan, Yuan, Lin: "Sulforaphane induces p53‑deficient SW480 cell apoptosis via the ROS‑MAPK signaling pathway." in: Molecular medicine reports, Vol. 16, Issue 5, pp. 7796-7804, (2018) (PubMed).

    Popp, Tran, Patel, Segatori: "Autophagic response to cellular exposure to titanium dioxide nanoparticles." in: Acta biomaterialia, Vol. 79, pp. 354-363, (2018) (PubMed).

  • Target

    IgG

    Substanzklasse

    Antibody

    Hintergrund

    Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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