Esel anti-Maus IgG Antikörper (DyLight 488) - Preadsorbed

Produktnummer ABIN6699007

Hersteller Produkt- Nr.: 610-741-124

Kurzübersicht für Esel anti-Maus IgG Antikörper (DyLight 488) - Preadsorbed (ABIN6699007)

Target: IgG

Klonalität: Polyklonal

Konjugat: DyLight 488

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotyp: IgG

Produktdetails

Reaktivität: Maus

Wirt: Esel

Hersteller Produkt- Nr.: 610-741-124

Hersteller: Rockland

Verwendungszweck: Mouse IgG (H&L) Antibody DyLight™ 488 Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins

Produktmerkmale: Donkey anti-Mouse IgG DyLight 488™ Conjugated Antibody, Donkey anti Mouse IgG Antibody DyLight 488™ Conjugation,Anti-Mouse IgG DyLight488 Antibody generated in donkey detects reactivity to Mouse IgG.

Aufreinigung: Preadsorption: Pre-Adsorbed

Immunogen: Mouse IgG whole molecule

Labeling Ratio: 3.8

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Kommentare:

Anti-Mouse IgG DyLight488 Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, IHC, Multiplex

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Zukowski, Sannella, Rockhold, Kalantar, Yu, SantaCruz-Calvo, Kuhn, Hah, Ouyang, Wang, Murphy, Marszalkowski, Gibney, Drummond, Proctor, Hasturk, Nikolajczyk, Bharath: "STAT3 modulates CD4+ T mitochondrial dynamics and function in aging." in: Aging cell, Vol. 22, Issue 11, pp. e13996, (2023) (PubMed).

Conway, Rockhold, SantaCruz-Calvo, Zukowski, Pugh, Hasturk, Kern, Nikolajczyk, Bharath: "Obesity and Fatty Acids Promote Mitochondrial Translocation of STAT3 Through ROS-Dependent Mechanisms." in: Frontiers in aging, Vol. 3, pp. 924003, (2022) (PubMed).

Bharath, Agrawal, McCambridge, Nicholas, Hasturk, Liu, Jiang, Liu, Guo, Deeney, Apovian, Snyder-Cappione, Hawk, Fleeman, Pihl, Thompson, Belkina, Cui, Proctor, Kern, Nikolajczyk: "Metformin Enhances Autophagy and Normalizes Mitochondrial Function to Alleviate Aging-Associated Inflammation." in: Cell metabolism, Vol. 32, Issue 1, pp. 44-55.e6, (2021) (PubMed).

Golenberg, Squirrell, Bennin, Rindy, Pistono, Eliceiri, Shelef, Kang, Huttenlocher: "Citrullination regulates wound responses and tissue regeneration in zebrafish." in: The Journal of cell biology, Vol. 219, Issue 4, (2020) (PubMed).

Watanabe, Hiramatsu, Nishimura, Ono: "Glucagon-like Peptide-1 Receptor Expression in the Pancreatic D Cells of Three Avian Species; White Leghorn Chickens, Northern Bobwhites, and Common Ostriches." in: The journal of poultry science, Vol. 55, Issue 3, pp. 199-203, (2018) (PubMed).

De Luigi, Mariani, De Paola, Re Depaolini, Colombo, Russo, Rondelli, Brocca, Adler-Abramovich, Gazit, Del Favero, Cantù, Salmona: "Doxycycline hinders phenylalanine fibril assemblies revealing a potential novel therapeutic approach in phenylketonuria." in: Scientific reports, Vol. 5, pp. 15902, (2016) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.