Ziege anti-Maus IgG Antikörper (Cy5.5) - Preadsorbed

Produktnummer ABIN6698988

Hersteller Produkt- Nr.: 610-113-121

Kurzübersicht für Ziege anti-Maus IgG Antikörper (Cy5.5) - Preadsorbed (ABIN6698988)

Target: IgG

Klonalität: Polyklonal

Konjugat: Cy5.5

Applikation: Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotyp: IgG

Produktdetails

Reaktivität: Maus

Wirt: Ziege

Hersteller Produkt- Nr.: 610-113-121

Hersteller: Rockland

Verwendungszweck: Mouse IgG (H&L) Antibody CY5.5 Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins

Produktmerkmale: Goat Anti-Mouse IgG (H&L) Antibody CY5.5 Conjugated Pre-Adsorbed, Goat Anti Mouse IgG Antibody CY5.5 Conjugated,Anti-Mouse IgG Cy5.5 Antibody generated in goat detects reactivity to Mouse IgG.

Aufreinigung: Preadsorption: Pre-Adsorbed

Immunogen: Mouse IgG whole molecule

Labeling Ratio: 4.3

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: 1:10,000 - 1:50,000

Flow_Cytometry_Dilution: 1:500 - 1:2,500

IF_Microscopy_Dilution: 1:1,000 - 1:5,000

Other: FLOW CYTOMETRY 1:500 - 1:2,500

Kommentare:

Anti-Mouse IgG Cy5.5 Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suggested Applications: IF

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Gawronska-Kozak, Kopcewicz, Machcinska-Zielinska, Walendzik, Wisniewska, Drukała, Wasniewski, Rutkowska, Malinowski, Pulinski: "Gender Differences in Post-Operative Human Skin." in: Biomedicines, Vol. 11, Issue 10, (2023) (PubMed).

Machcinska, Walendzik, Kopcewicz, Wisniewska, Rokka, Pääkkönen, Slowinska, Gawronska-Kozak: "Hypoxia reveals a new function of Foxn1 in the keratinocyte antioxidant defense system." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 36, Issue 8, pp. e22436, (2022) (PubMed).

Machcinska, Kopcewicz, Bukowska, Walendzik, Gawronska-Kozak: "Impairment of the Hif-1α regulatory pathway in Foxn1-deficient (Foxn1-/- ) mice affects the skin wound healing process." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 35, Issue 2, pp. e21289, (2021) (PubMed).

Kur-Piotrowska, Bukowska, Kopcewicz, Dietrich, Nynca, Slowinska, Gawronska-Kozak: "Foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing." in: Scientific reports, Vol. 8, Issue 1, pp. 5425, (2018) (PubMed).

Yamashita, Ancillotti, Rangel, Fontenele, Figueiredo-Freitas, Possidonio, Soares, Sorenson, Mermelstein, Nogueira: "Balance between S-nitrosylation and denitrosylation modulates myoblast proliferation independently of soluble guanylyl cyclase activation." in: American journal of physiology. Cell physiology, Vol. 313, Issue 1, pp. C11-C26, (2017) (PubMed).

Markoullis, Bulian, Hölzlwimmer, Quintanilla-Martinez, Heiliger, Zitzelsberger, Scherb, Mysliwietz, Uphoff, Drexler, Adler, Busch, Schmidt, Mahabir: "Mycoplasma contamination of murine embryonic stem cells affects cell parameters, germline transmission and chimeric progeny." in: Transgenic research, Vol. 18, Issue 1, pp. 71-87, (2009) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.