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Ziege anti-Maus IgG Antikörper (Cy3) - Preadsorbed

WB, FACS, FLISA, FM Polyclonal IgG Cy3
Rockland
Produktnummer ABIN6698986
Hersteller Produkt- Nr.: 610-104-121

Kurzübersicht für Ziege anti-Maus IgG Antikörper (Cy3) - Preadsorbed (ABIN6698986)

Target

IgG

Klonalität

  • 3453
  • 231
  • 16
  • 1
Polyklonal

Konjugat

  • 666
  • 423
  • 422
  • 400
  • 274
  • 262
  • 235
  • 119
  • 105
  • 72
  • 67
  • 64
  • 61
  • 58
  • 58
  • 49
  • 44
  • 43
  • 40
  • 40
  • 39
  • 39
  • 36
  • 36
  • 34
  • 32
  • 31
  • 30
  • 29
  • 29
  • 29
  • 29
  • 29
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 27
  • 26
  • 26
  • 25
  • 21
  • 21
  • 19
  • 17
  • 17
  • 16
Cy3

Applikation

  • 2120
  • 2053
  • 1899
  • 1873
  • 999
  • 919
  • 895
  • 520
  • 424
  • 234
  • 234
  • 149
  • 102
  • 98
  • 90
  • 84
  • 51
  • 33
  • 30
  • 28
  • 25
  • 20
  • 15
  • 11
  • 9
  • 8
  • 7
  • 7
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Isotyp

IgG
  • Reaktivität

    • 752
    • 705
    • 609
    • 474
    • 410
    • 244
    • 213
    • 176
    • 146
    • 141
    • 124
    • 110
    • 107
    • 101
    • 83
    • 62
    • 48
    • 33
    • 28
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus

    Wirt

    • 1786
    • 1583
    • 569
    • 284
    • 122
    • 112
    • 41
    • 32
    • 22
    • 21
    • 15
    • 11
    • 11
    Ziege

    Hersteller Produkt- Nr.

    610-104-121

    Hersteller

    Rockland

    Verwendungszweck

    Mouse IgG (H&L) Antibody CY3 Conjugated Pre-Adsorbed

    Kreuzreaktivität (Details)

    Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins

    Produktmerkmale

    Goat Anti-Mouse IgG (H&L) Antibody CY3 Conjugated Pre-Adsorbed, Goat Anti Mouse IgG Antibody CY3 Conjugated,Anti-Mouse IgG Cy3 Antibody generated in goat detects reactivity to Mouse IgG.

    Aufreinigung

    Preadsorption: Pre-Adsorbed

    Immunogen

    Mouse IgG whole molecule

    Labeling Ratio

    8.5
  • Applikationshinweise

    FLISA_Dilution: 1:10,000 - 1:50,000

    Flow_Cytometry_Dilution: 1:500 - 1:2,500

    IF_Microscopy_Dilution: 1:1,000 - 1:5,000

    Other: FLOW CYTOMETRY 1:500 - 1:2,500

    Kommentare

    Anti-Mouse IgG Cy3 Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Suggested Applications: IF, IHC, Multiplex

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration

    1.0 mg/mL

    Buffer

    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Ng-Blichfeldt, Stewart, Clatworthy, Williams, Röper: "Identification of a core transcriptional program driving the human renal mesenchymal-to-epithelial transition." in: Developmental cell, Vol. 59, Issue 5, pp. 595-612.e8, (2024) (PubMed).

    Hartmann, Raabe, Wenisch, Arnhold: "Amniotic fluid derived stem cells give rise to neuron-like cells without a further differentiation potential into retina-like cells." in: American journal of stem cells, Vol. 2, Issue 2, pp. 108-18, (2013) (PubMed).

    Venkatesan, Natarajan, Schwarz, Mayer, Alpadi, Magupalli, Sung, Schmitz: "Nicotinamide adenine dinucleotide-dependent binding of the neuronal Ca2+ sensor protein GCAP2 to photoreceptor synaptic ribbons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 19, pp. 6559-76, (2010) (PubMed).

    Arnhold, Post, Glüer, Hoopmann, Wenisch, Volpers, Addicks: "Neuronal characteristics of amniotic fluid derived cells after adenoviral transformation." in: Cell biology international, Vol. 32, Issue 12, pp. 1559-66, (2009) (PubMed).

    Pyo, Sui, Dhume, Palomeque, Blaxall, Diaz, Tunstead, Logothetis, Hajjar, Schecter: "CXCR4 modulates contractility in adult cardiac myocytes." in: Journal of molecular and cellular cardiology, Vol. 41, Issue 5, pp. 834-44, (2007) (PubMed).

    Arnhold, Klein, Klinz, Absenger, Schmidt, Schinköthe, Brixius, Kozlowski, Desai, Bloch, Addicks: "Human bone marrow stroma cells display certain neural characteristics and integrate in the subventricular compartment after injection into the liquor system." in: European journal of cell biology, Vol. 85, Issue 6, pp. 551-65, (2007) (PubMed).

    Wolter, Tauer, Fuchs, Volk: "Mapping of cytoskeletal components in the hippocampal formation of the tree shrew (Tupaia belangeri)." in: Journal of chemical neuroanatomy, Vol. 17, Issue 2, pp. 65-74, (1999) (PubMed).

  • Target

    IgG

    Substanzklasse

    Antibody

    Hintergrund

    Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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