Schaf anti-Maus IgG Antikörper (Cy2)

Produktnummer ABIN6698983

Hersteller Produkt- Nr.: 610-611-002

Kurzübersicht für Schaf anti-Maus IgG Antikörper (Cy2) (ABIN6698983)

Target: IgG

Klonalität: Polyklonal

Konjugat: Cy2

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Isotyp: IgG

Produktdetails

Reaktivität: Maus

Wirt: Schaf

Hersteller Produkt- Nr.: 610-611-002

Hersteller: Rockland

Verwendungszweck: Mouse IgG (H&L) Antibody Cy2 Conjugated

Produktmerkmale: Sheep Anti-Mouse IgG (H&L) Antibody CY2 Conjugated, Sheep Anti Mouse IgG Antibody CY2 Conjugated,Anti-Mouse IgG Cy2 Antibody generated in sheep detects reactivity to Mouse IgG.

Immunogen: Mouse IgG whole molecule

Labeling Ratio: 3.6

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: 1:10,000 - 1:50,000

ELISA_Dilution: User Optimized

Flow_Cytometry_Dilution: 1:500 - 1:2,500

IF_Microscopy_Dilution: 1:1,000 - 1:5,000

Western_Blot_Dilution: User Optimized

Kommentare:

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suggested Applications: IF

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.