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Ziege anti-Maus IgG Antikörper (Cy2) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG Cy2 F(ab')2 fragment
Rockland
Produktnummer ABIN6698833
Hersteller Produkt- Nr.: 710-111-032
  • Target Alle IgG Produkte
    IgG
    Fragment
    F(ab')2 fragment
    Reaktivität
    • 730
    • 695
    • 598
    • 468
    • 399
    • 240
    • 208
    • 175
    • 145
    • 137
    • 112
    • 107
    • 106
    • 101
    • 84
    • 58
    • 48
    • 34
    • 28
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Maus
    Wirt
    • 1775
    • 1547
    • 569
    • 261
    • 112
    • 111
    • 32
    • 31
    • 21
    • 18
    • 15
    • 11
    • 11
    Ziege
    Klonalität
    • 3406
    • 182
    • 16
    • 1
    Polyklonal
    Konjugat
    • 592
    • 422
    • 422
    • 380
    • 274
    • 262
    • 235
    • 119
    • 106
    • 72
    • 67
    • 64
    • 61
    • 58
    • 58
    • 50
    • 44
    • 43
    • 40
    • 40
    • 39
    • 39
    • 36
    • 36
    • 34
    • 32
    • 31
    • 30
    • 29
    • 29
    • 29
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 21
    • 21
    • 19
    • 17
    • 17
    • 16
    Cy2
    Applikation
    • 2062
    • 1988
    • 1864
    • 1807
    • 998
    • 919
    • 895
    • 487
    • 424
    • 234
    • 234
    • 149
    • 98
    • 84
    • 65
    • 58
    • 51
    • 30
    • 28
    • 25
    • 20
    • 15
    • 11
    • 9
    • 8
    • 7
    • 7
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Hersteller Produkt- Nr.
    710-111-032
    Hersteller
    Rockland
    Verwendungszweck
    F(ab')2 Mouse IgG (H&L) Antibody Cy2 Conjugated Pre-Adsorbed
    Kreuzreaktivität (Details)
    Minimal crossreactivity against Bv Hs Hu Rb Rt & Sh Serum Proteins
    Produktmerkmale
    Goat F(ab')2 Anti-Mouse IgG Antibody CY2 Conjugation, Goat Fab2 Anti-Mouse IgG CY2 Conjugated Antibody,F(ab')2 Anti-Mouse IgG (H&L) Cy2 Antibody generated in goat was generated by enzymatic cleavage and subsequent separation from the Fc fragment.
    Aufreinigung
    Preadsorption: Pre-Adsorbed
    Immunogen
    Mouse IgG whole molecule
    Isotyp
    IgG
    Labeling Ratio
    1.9
  • Applikationshinweise

    FLISA_Dilution: 1:10,000 - 1:50,000

    Flow_Cytometry_Dilution: 1:500 - 1:2,500

    IF_Microscopy_Dilution: 1:1,000 - 1:5,000

    Other: FLOW CYTOMETRY 1:500 - 1:2,500

    Kommentare

    Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
    Hintergrund
    Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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