Ziege anti-Maus IgG (Heavy & Light Chain) Antikörper (HRP)

Produktnummer ABIN101766

Hersteller Produkt- Nr.: 610-103-121

Kurzübersicht für Ziege anti-Maus IgG (Heavy & Light Chain) Antikörper (HRP) (ABIN101766)

Target: IgG

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Maus

Wirt: Ziege

Hersteller Produkt- Nr.: 610-103-121

Hersteller: Rockland

Verwendungszweck: Mouse IgG (H&L) Antibody Peroxidase Conjugated Pre-adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

Produktmerkmale: Anti-Mouse IgG and IgM whole molecule peroxidase conjugated antibody generated in goat detects specifically Mouse IgG and IgM whole molecule. This secondary peroxidase conjugated antibody anti-mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Aufreinigung: HRP secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Mouse IgG whole molecule

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Mouse IgG Peroxidase Antibody has been tested by ELISA and western blot and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:20,000 ELISA Dilution: 1:180,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store secondary antibody at 4° C prior to restoration.   For extended storage aliquot antibody and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Anti-Mouse IgG HRP secondary antibody is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Lobo-Jarne, Nývltová, Pérez-Pérez, Timón-Gómez, Molinié, Choi, Mourier, Fontanesi, Ugalde, Barrientos: "Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics." in: Cell reports, Vol. 25, Issue 7, pp. 1786-1799.e4, (2018) (PubMed).

Fan, Tan, Li, Zhao, Yuan, Liu, Wang, Zhang: "Upregulation of miR‑185 promotes apoptosis of the human gastric cancer cell line MGC803." in: Molecular medicine reports, Vol. 17, Issue 2, pp. 3115-3122, (2018) (PubMed).

Ercan, Han, Di Nardo, Winden, Han, Hoyo, Saffari, Leask, Geschwind, Sahin: "Neuronal CTGF/CCN2 negatively regulates myelination in a mouse model of tuberous sclerosis complex." in: The Journal of experimental medicine, Vol. 214, Issue 3, pp. 681-697, (2017) (PubMed).

Lampi, Faber, Huynh, Bordeleau, Zanotelli, Reinhart-King: "Simvastatin Ameliorates Matrix Stiffness-Mediated Endothelial Monolayer Disruption." in: PLoS ONE, Vol. 11, Issue 1, pp. e0147033, (2016) (PubMed).

Dickerman, White, Kessler, Sadler, Williams, Sen: "The protein activator of protein kinase R, PACT/RAX, negatively regulates protein kinase R during mouse anterior pituitary development." in: The FEBS journal, Vol. 282, Issue 24, pp. 4766-81, (2016) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.